目的·检测白介素（interleukin，IL） -1β、IL-6和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）在种植体周围炎患者龈沟液中的表达并分析其临床意义。方法·选择因牙列缺损行种植修复的患者24例，其中种植周围炎组患者12例，种植体周围组织健康组12例；另选择12名具健康天然牙的健康者为对照组。收集3组患者龈沟液，采用酶联免疫吸附法检测龈沟液中IL-1β、IL-6和TNF-α含量，分析其表达水平与龈沟液量和牙周探诊深度的相关性。结果·种植体周围炎组患者IL-1β、IL-6和TNF-α明显高于对照组（均P<0.05），且种植体周围炎组IL-1β和IL-6表达水平显著高于种植体周围组织健康组（均P<0.05）；其中种植体周围炎组龈沟液中TNF-α表达水平与探诊深度呈正相关（r=0.600，P=0.039）。结论·种植体周围炎患者龈沟液中IL-1β、IL-6和TNF-α表达升高，且TNF-α表达水平与探诊深度呈正相关。

Objective · To investigate expressions of interleukin (IL) -1β, IL-6 and tumor necrosis factor-α (TNF-α) in sulcular fluid of peri-implantitis. Methods · Twenty-four patients with implant restoration were selected, including 12 patients in the peri-implantitis group and 12 patients in the peri-implant health group. Twelve healthy persons with healthy gingival tissues were taken as the control group. The levels of IL-1β, IL-6 and TNF-α in gingival crevicular fluid were detected by enzyme-linked immunosorbent assay. The correlations between the expressions of these cytokines and the amount of gingival crevicular fluid or the probing depth were analyzed. Results · The levels of IL-1β, IL-6 and TNF-α in the peri-implantitis group were significantly higher than those in the control group (all P<0.05). The expression levels of IL-1β and IL-6 in the peri-implantitis group were significantly higher than those in the peri-implant health group (both P<0.05). The level of TNF-α in the peri-implantitis group was positively correlated with the probing depth (r=0.600, P=0.039). Conclusion · The levels of IL-1β, IL-6 and TNF-α in gingival crevicular fluid are highly expressed in patients with peri-implantitis. The expression of TNF-α is positively correlated with the probing depth.