上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2021 , Vol. 41 >Issue 11: 1429 - 1435

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.11.005

论著 · 基础研究

差异表达微RNA作为多囊卵巢综合征生物标志物的研究

  • 王昱欢 ,
  • 丁奕岑 ,
  • 蔡瑶雨 ,
  • 康亚妮
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  • 1.上海交通大学生物医学工程学院，上海 200240
    2.上海交通大学医学院，上海 200025
王昱欢（1999—），女，本科生；电子信箱：wyh1999@sjtu.edu.cn。
康亚妮，电子信箱：kangyani@sjtu.edu.cn。

网络出版日期: 2021-12-03

基金资助

上海市自然科学基金(19ZR1476100);转化医学国家重大科技基础设施（上海）课题(TMSK-2020-109);医工交叉研究基金(YG2019GD02)

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Study on differentially expressed microRNA as a biomarker of polycystic ovary syndrome

  • Yu-huan WANG ,
  • Yi-cen DING ,
  • Yao-yu CAI ,
  • Ya-ni KANG
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  • 1.Shanghai Jiao Tong University School of Biomedical Engineering, Shanghai 200240, China
    2.Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
KANG Ya-ni, E-mail: kangyani@sjtu.edu.cn.

Online published: 2021-12-03

Supported by

Natural Science Foundation of Shanghai(19ZR1476100);National Infrastructures for Translational Medicine (Shanghai)(TMSK-2020-109);Interdisciplinary Program of Medical Engineering Cross Fund(YG2019GD02)

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摘要

目的·探索可作为多囊卵巢综合征（polycystic ovary syndrome，PCOS）生物标志物的微RNA（microRNA，miRNA）及其在PCOS发病机制中的生物学意义。方法·选取上海交通大学医学院附属仁济医院2019年1月—10月收治的5例PCOS患者为观察组，另选取5例体检健康女性作为对照组。采集2组的颗粒细胞，用TRIzol法从中提取RNA，利用TruSeq Small RNA Library Prep试剂盒构建miRNA-seq文库，文库质检合格后用NextSeq500进行高通量测序，对测序结果进行生物信息学数据分析。采用RT-qPCR实验检验候选miRNA标志物是否存在差异表达。结果·分析获得颗粒细胞中20条差异表达miRNA（均P<0.05），其中miR-196a-5p、miR-10a-5p和miR-451a表达显著上调，miR-877-5p和miR-2355-5p表达显著下调，这些差异表达miRNA及其靶基因可能参与PCOS发生发展的转录调控机制。结论·差异表达miRNA具有作为PCOS生物标志物的潜能，并参与PCOS的发生发展。

关键词： 微RNA; 生物标志物; 多囊卵巢综合征; 基因表达; 靶基因

本文引用格式

王昱欢 , 丁奕岑 , 蔡瑶雨 , 康亚妮 . 差异表达微RNA作为多囊卵巢综合征生物标志物的研究[J]. 上海交通大学学报（医学版）, 2021 , 41(11) : 1429 -1435 . DOI: 10.3969/j.issn.1674-8115.2021.11.005

Abstract

Objective

·To explore microRNAs (miRNAs) as biomarkers of polycystic ovary syndrome (PCOS) and the biological significance in the pathogenesis of PCOS.

Methods

·Five patients with PCOS from January 2019 to October 2019 in Renji Hospital, Shanghai Jiao Tong University School of Medicine were selected as the observation group, and 5 healthy women were selected as the control group. Granulosa cells from 2 groups were collected and RNA was extracted by TRIzol method. TruSeq Small RNA Library Prep Kit was used to construct miRNA-Seq library. After the quality inspection of the library, NextSeq500 was used for high-throughput sequencing. Bioinformatics data analysis was performed on the sequencing results. RT-qPCR was used to verify the differential expression of candidate miRNA markers.

Results

·We finally got 20 differentially expressed miRNAs (all P<0.05). Among them, miR-196a-5p, miR-10a-5p and miR-451a were significantly up-regulated, while miR-877-5p and miR-2355-5p were significantly down-regulated. These differentially expressed miRNAs and their target genes may be involved in the transcriptional regulation mechanism of PCOS occurrence and development.

Conclusion

·Differentially expressed miRNAs have potential as PCOS biomarkers and are involved in the occurrence and development of PCOS.

Key words： microRNA (miRNA); biomarker; polycystic ovary syndrome; gene expression; target gene

参考文献

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