目的·建立一种用于研究性腺发育及相关疾病的转基因斑马鱼品系Tg（amh:mCherry），并对斑马鱼性腺的发育过程进行示踪分析。方法·使用斑马鱼抗米勒管激素（anti-Müllerian hormone，amh）基因编码区的上游启动子序列和红色荧光蛋白的基因编码序列构建重组转基因表达载体pTol2-amh-mCherry，并采用Sanger测序对构建的载体进行验证。将上述载体和Tol2转座酶的mRNA显微共注射入斑马鱼胚胎，使用荧光显微镜挑选带有荧光的斑马鱼，筛选出能够稳定遗传的转基因斑马鱼品系Tg（amh:mCherry），并通过原位杂交技术检测荧光信号的位置与amh内源性表达位置是否一致。采用荧光显微镜观察F3代转基因斑马鱼的红色荧光情况，并对其性腺发育过程进行追踪和分析。结果·成功构建了pTol2-amh-mCherry载体，并建立了能稳定遗传的F3代转基因斑马鱼品系Tg（amh:mCherry）。原位杂交技术的结果显示，该转基因斑马鱼的红色荧光信号位置与内源性amh表达位置一致。通过对F3代转基因斑马鱼荧光的观察和分析后发现，斑马鱼性腺的发育存在5种情况。结论·Tg（amh:mCherry）转基因斑马鱼品系的成功构建有利于对斑马鱼性腺发育的示踪分析，也为性腺相关疾病的研究提供了较理想的实验模型。

Objective ·To establish a transgenic zebrafish line Tg(amh:mCherry) for studying gonadal development and related diseases, and to trace and analyze the developmental process of zebrafish gonads. Methods ·A recombinant transgenic expression vector pTol2-amh-mCherry was constructed by using the upstream promoter sequence of the zebrafish anti-Müllerian hormone (amh) gene coding region and the gene coding sequence of the red fluorescent protein, and was validated by using Sanger sequencing. The recombinant transgenic expression vector and mRNA of Tol2 transposase were co-microinjected into zebrafish embryos, and fluorescent zebrafish were selected by using fluorescence microscope to screen for a stable inherited transgenic zebrafish line Tg(amh:mCherry). In situ hybridization was used to detect whether the location of the fluorescence signals was consistent with the location of endogenous expression of amh. The red fluorescence of transgenic zebrafish from generation F3 was observed by fluorescence microscopy, and the process of gonadal development was tracked and analyzed. Results ·The recombinant transgenic expression vector pTol2-amh-mCherry was successfully constructed, and a stable inherited F3 generation transgenic zebrafish line Tg(amh:mCherry) was successfully established. The results of in situ hybridization showed that the location of the red fluorescence signals in the transgenic zebrafish Tg(amh:mCherry) was consistent with the location of endogenous expression of amh. Through observation and analysis of the fluorescence of transgenic zebrafish from F3 generation, five developmental patterns of zebrafish gonads were found. Conclusion ·The successful construction of Tg(amh: mCherry) transgenic zebrafish line facilitates the tracer analysis of zebrafish gonadal development, which provides a better experimental model for the study of gonad-related diseases.