目的 采用蛋白质组学技术研究急性运动轴索型神经病（AMAN）患者血清对大鼠脊髓运动神经元凋亡相关蛋白表达的影响。方法 分别以16例AMAN患者和16名健康献血者的血清对原代培养大鼠脊髓运动神经元进行干预,24 h后行双向凝胶电泳，MALDI-TOF/TOF串联质谱鉴定神经元发生变化的蛋白，表达水平上升或下降超过30%的点判定为差异蛋白质点。结果 AMAN患者血清干预与健康献血者血清干预的大鼠脊髓运动神经元比较，有21种差异表达蛋白，其中内质网蛋白29、不均一核糖核蛋白H、蛋白酶体α亚基1、3-磷酸甘油醛脱氢酶、电压门控阴离子选择性通道蛋白1为凋亡相关蛋白。结论 在AMAN患者血清作用下，大鼠脊髓运动神经元的细胞凋亡相关蛋白表达发生了变化。

Objective To investigate the effects of serum of patients with acute motor axonal neuropathy(AMAN) on expression of apoptosis-related proteins in rat spinal motor neuron with proteomics. Methods Serum samples of 16 patients with AMAN and 16 healthy people were obtained, and two-dimensional gel electrophoresis was performed 24 h after primary culture of rat spinal motor neuron. The proteins of interest were identified by MALDI-TOF/TOF tandem mass spectrometry, and differential protein spots were defined as those with changes of expression >30%. Results There were 21 differentially expressed proteins, among which heterogeneous nuclear ribonucleoprotein H, proteasome subunit alpha type 1, endoplasmic reticulum protein 29, glyceraldehyde-3-phosphate dehydrogenase and voltagedependent anion-selective channel protein 1 were apoptosis-related proteins. Conclusion Serum of patients with AMAN may lead to changes of expression of apoptosis-related proteins in rat spinal motor neuron.