›› 2013, Vol. 33 ›› Issue (5): 655-.doi: 10.3969/j.issn.1674-8115.2013.05.029

• Original article (Clinical research) • Previous Articles     Next Articles

Expression of ApoA-Ⅰ in granulosa cells in patients with polycystic ovary syndrome

TONG La-juan, QIU Yan, MENG Jiang-ping, XING Gui-bang   

  1. Department of Assistant Reproductive Centre, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
  • Online:2013-05-28 Published:2013-05-28

Abstract:

Objective To investigate the expression of apolipoprotein A-Ⅰ (ApoA-Ⅰ) in granulosa cells (GCs) of patients with polycystic ovary syndrome (PCOS). Methods Forty women undergoing in vitro fertilization and embryo transfer were divided into PCOS group (n=20) and control group (n=20), and serum samples on preoperative examination day and GCs samples were collected. The expression of ApoA-Ⅰ protein in GCs was detected by Western blotting, the expression of ApoA-Ⅰ mRNA in GCs was determined by Real-time PCR, and the serum level of ApoA-Ⅰ was measured by immunoturbidimetry. Results The expression of ApoA-Ⅰ protein and mRNA in GCs in PCOS group was significantly lower than that in control group (P<0.001, P<0.01), and the serum level of ApoA-Ⅰ in PCOS group was also significantly lower than that in control group (P<0.05). Conclusion The expression of ApoA-Ⅰ is down-regulated in GCs in patients with PCOS.

Key words: polycystic ovary syndrome, apolipoprotein A-Ⅰ, granulosa cells