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Therapeutic effect of TNF-α monoclonal antibody on rats with allergic rhinitis and relevant mechanisms

TANG Qiao-fei1, ZHANG Shuang1, CHENG Yang2, TAI Xu-hui3, JIANG Yu-qiu1, YAN Zhi-yong1   

  1. 1.Department of ENT, the Second Affiliated Hospital of Shenyang Medical College, Shenyang 110002, China; 2.Department of Pathophysiology, Shenyang Medical College, Shenyang 110034, China; 3.PLA 463 Hospital of Shenyang Military Command, Shenyang 110042, China
  • Online:2015-09-28 Published:2015-09-30
  • Supported by:

    Project of Shenyang Medical College, F14-158-9-23; Scientific Research Foundation of Shenyang Medical College, 200323

Abstract:

Objective  To explore the therapeutic effect of the intervention of TNF-α monoclonal antibody on rats with allergic rhinitis (AR) and the underlying molecular mechanism. Methods  Eighteen BALB/c rats were randomly divided into the control group, AR model group, and TNF-α antibody intervention group. The ovalbumin (OVA) induced AR model was established and TNF-α monoclonal antibody was dropped nasally before nasal cavity was stimulated. Behavioral scores of mice were obtained and histopathologic changes of nasal mucosa were observed by H-E staining. The apoptosis of cells was detected by the TUNEL method. Expression levels of TNF-α, IL-1β, and IgE in nasal mucosa fluid were detected with ELISA. Changes of expressions of NF-κB p65, IκB, and apoptosis-related proteins were measured by Western blotting. Results  Symptoms of AR and inflammatory response of the TNF-α antibody intervention group alleviated. Compared with the AR model group, expression levels of TNF-α, IL-1β, and IgE in nasal mucosa fluid and NF-κB p65, cleaved caspase-3, and Bax in nasal mucosa tissues decreased significantly, but expression levels of IκB and Bcl-2 increased. Conclusion  The intervention of TNF-α monoclonal antibody has certain therapeutic effect on rats with allergic rhinitis by inhibiting the activation of NF-κB signaling pathway, regulating Th1/Th2 balance, decreasing expression levels of inflammatory factors, and inhibiting apoptosis of cells.

Key words: TNF-&alpha, monoclonal antibody, allergic rhinitis, NF-κB signaling pathway