Abstract:

Objective  To observe the effect of dexmedetomidine on the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in lung tissues of rats with sepsis. Methods  Sixty male Sprague Dawley (SD) rats were randomly divided into the sham group (n=15), sepsis group (n=15), dexmedetomidine group (n=15), and yohimbine+ dexmedetomidine group (n=15). The sepsis model was established by cecal ligation and puncture (CLP). The survival rate of rats in 24 h was observed. Serum IL-6, TNF-α, and sTREM-1 levels were measured by enzyme-linked immunosorbent assay (ELISA). The pathological changes of lung tissues were observed and scored. The expression of TREM-1 in lung tissues was detected by real-time PCR and immunohistochemichal method. Results  Compared with the sepsis group, the mortality rate and serum IL-6, TNF-α, and sTREM-1 levels of dexmedetomidine group remarkably decreased (P<0.05). The inflammatory response of lung tissue significantly alleviated (P<0.05) and the expression of TREM-1 remarkably decreased (P<0.05). Conclusion  Dexmedetomidine can alleviate the inflammatory response of lung tissues of rats with sepsis by inhibiting the expression of TREM-1.

Key words: dexmedetomidine, triggering receptor expressed on myeloid cells-1, sepsis, inflammatory