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Effects of adiponectin on the proliferation and apoptosis of mesenchymal stem cells

FU Zong-jie1, LIN Yuan1, MIAO Jie1, YANG Guo-yuan2,3, SHEN Lin-hui1   

  1. 1.Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China; 3.Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Natural Science Foundation of China， 81300725

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Abstract:

Objective To explore the effects of adiponectin (Apn) on the proliferation and apoptosis of mesenchymal stem cells (MSCs). Methods Primary rat MSCs were separated and cultured. MSCs were transfected with constructed mouse Apn gene recombinant lentivirus (Lenti-Apn-IRES-EGFP）or control lentivirus (Lenti-IRES-EGFP) and the transfection efficiency was measured. The in vitro proliferation of Gfp-MSCs and Apn-MSCs were detected with the CCK8 kit after transfection. The apoptosis rate was measured with the flow cytometer 12 h after the construction of oxygen and glucose deprivation (OGD) model. Results The proliferation of Apn-MSCs was faster as compared with Gfp-MSCs (P＝0.001). The apoptosis rate of Apn-MSCs was lower than that of Gfp-MSCs 12 h after the construction of OGD model (P＝0.01). Conclusion Apn can promote the proliferation of MSCs and reduce the apoptosis of MSCs after oxygen and glucose deprivation.

Key words: adiponectin; , mesenchymal stem cells; , apoptosis; , proliferation