›› 2018, Vol. 38 ›› Issue (1): 42-.doi: 10.3969/j.issn.1674-8115.2018.01.008

• Original article (Basic research) • Previous Articles     Next Articles

Effect of gold nanoparticle on autophagy flux and exosome secretion of mesenchymal stem cells

YAN Chun1, MA Yu-jie2, XIE Wei-yue1, YANG Qing-yuan3, SUN Feng-yong3, YANG Zhi2, XUE Song1, LIAN Feng1   

  1. 1. Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127; 2. Ministry of Education Key Laboratory of Thin Film and Microfabrication Technology, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240; 3. Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai 200072
  • Online:2018-01-28 Published:2018-03-09
  • Supported by:
    Shanghai Jiao Tong University Medicine-Engineering Foundation, YG2015MS51

PDF (PC)

1078

Abstract: Objective · To investigate the effect of gold nanoparticles (AuNPs) on exosome secretion of mesenchymal stem cells (MSCs) via blocking
autophagy flux. Methods · Rat derived MSCs were treated with AuNPs (0, 15, 30, 90, 150 μg/mL), and the cell proliferation was detected with CCK-8
assay to evaluate the cytotoxicity of AuNPs. Then, MSCs were divided into normal control group, AuNPs group (20 μg/mL AuNPs for 72 h) and positive
control group (5 μmol/L chloroquine for 4 h). Transmission electron microscopy was used to observe the ultrastructure of MSCs, and exosomes were
extracted by ultracentrifugation and tested with nanoparticle analyzer and scan electron microscopy. The autophagy was observed by confocal microscopy
and verified by Western blotting. Results · Low concentrations of AuNPs (0 to 30 μg/mL) slightly affected the cell proliferation. MSCs of AuNPs group
secreted more abundant and smaller exosomes than those of control group, and expressed significantly more LC3 and p62 (both P<0.05). Autophagosomes in AuNPs group were increased and fusion of lysosomes and autophagosomes was blocked. Conclusion · AuNPs block the autophagy flux and regulates the secretion of exosomes.

Key words: gold nanoparticle, mesenchymal stem cell, exosome, autophagy