›› 2018, Vol. 38 ›› Issue (8): 874-.doi: 10.3969/j.issn.1674-8115.2018.08.004

• Original article (Basic research) • Previous Articles     Next Articles

Effects of maternal vitamin D deficiency before and during pregnancy on intestinal flora and cathelicidin antimicrobial peptide in offspring rats

WANG Lei1, JIAO Xian-ting1, WEI Zhen-zhen1, LIU Bin3, LIU Xiao-yan3, YU Xiao-dan1, 2   

  1. 1. MOE-Shanghai Key Lab of Children′s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2. Department of Developmental and Behavioral Pediatrics, Shanghai Children′s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2018-08-28 Published:2018-09-11
  • Supported by:
    National Natural Science Foundation of China, 81773411, 81373004; National Key Research and Development Program of China, 2016YFC1305204; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20152220

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Abstract: Objective · To explore the effects of maternal vitamin D deficiency before and during pregnancy on the intestinal flora and cathelicidin antimicrobial peptide (CAMP) in offspring rats. Methods · Twenty-four female Sprague-Dawley rats (8-week-old) were randomly assigned to three groups (n8 per group), i.e. control group (C group), vitamin D deficiency group (VDD group), and vitamin D supplement group (VDS group). Special diets were used to build the rat models of vitamin D deficiency before and during pregnancy. Maternal 25(OH)D level was detectedliquid chromatography tandem mass spectrometry (LC-MS/MS) at gestational day 14.At the age of 4 weeks in offsprings, the level of 25(OH)D was measured; the faeces were collected for the detection of intestinal flora; the mRNA and protein of CAMP were evaluatedreal-time quantitative RTPCR and Western blotting, respectively. Results · The rat models of vitamin D deficiency before and during pregnancy were successfully established.The relative abundance of intestinal Lactobacillus in theoffspring rats in C group,VDD group andVDS group was 0.050±0.016,0.028±0.013 and0.033±0.021, respectively. Compared with C group, the relative abundance of intestinal Lactobacillus in VDD group significantly decreased (P<0.05)，and the mRNA and protein of colonic CAMPsignificantly decreased as well (P<0.05). Compared with VDD group, VDS group showed a trend of increase in the abundance of intestinal Lactobacillus;themRNA ofcolonic CAMPdidn’tchange significantly, but the proteinof colonic CAMP significantly increased (P<0.05). Conclusion · Vitamin D deficiency during pregnancy results in decreased abundance of intestinal Lactobacilli as well as reduced mRNAand protein of colonic CAMPin offspring rats. Vitamin D supplementation significantly improves the protein of colonic CAMP and induces a trend of increase in the abundance of intestinal Lactobacilli in offspring rats.

Key words: pregnancy, vitamin D, intestinal flora, cathelicidin antimicrobial peptide(CAMP)

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