›› 2010, Vol. 30 ›› Issue (12): 1509-.doi: 10.3969/j.issn.1674-8115.2010.12.015

• Original article (Basic research) • Previous Articles     Next Articles

Construction and self-activation of DLX4 in yeast two-hybrid system

QIAO Qin-qin, SUN Yun-yan, CHEN Ling-ling, LU Meng, FENG You-ji   

  1. Department of Obstetrics and Gynecology, The First Pepole's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
  • Online:2010-12-25 Published:2010-12-31
  • Supported by:

    National Natural Science Foundation of China, 30600674

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Abstract:

Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation. Methods Human placenta yeast-two cDNA bank was constructed, and was identified by PCR. DLX4 bait plasmids including pDEST32-DLX4(4-1C), pDEST32-DLX4(4-1N) and pDEST32-DLX4(4-2) were constructed and transfected into yeast MaV203 cells, and the self-activation effect was detected. Results It was verified by PCR that human placental cDNA bank was successfully constructed. pDEST32-DLX4(4-2) and pDEST32-DLX4(4-1C) had no self-activation effect, and could be used in the yeast two-hybrid system. Conclusion Yeast-two hybrid bank and DLX4 bait plasmids have been successfully constructed, and can be used to screen the protein interacting with DLX4.

Key words: DLX4, yeast two-hybrid, clone