›› 2010, Vol. 30 ›› Issue (3): 305-.

• Original article (Basic research) • Previous Articles     Next Articles

Expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase

MA Jue1, LI Lu1, GAO Xiao-hong1, WU Yu1, LU Xiang1, CHEN Qiu-ju2, SUN Xiao-xi1   

  1. 1. Department of Reproductive Medicine, International Peace Maternity &|Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China；2. Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    Shanghai Education Committee Foundation, 06BZ051

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Abstract:

Objective To investigate the expression of stromal cell-derived factor-1α (SDF-1α) and CXCR4 under different ovarian responses in human endometria of peri-implantation phase. Methods Endometria of peri-implantation phase (n=17) were collected, and were divided into moderate ovarian response group (n=6), high ovarian response group (n=6) and normal control group (n=5). The expression of SDF-1α/CXCR4 mRNA and protein in endometria of peri-implantation phase was detected and analysed by RT-PCR, Real-time PCR, immunohistochemistry and Western blotting. Results It was revealed by immunohistochemistry that CXCR4 was highly expressed in endometrial tissues, whereas SDF-1α was confined to glandular and celomic epithelial cells instead of stromal cells. There was no significant difference in the expression of SDF-1α/CXCR4 mRNA and protein in human endometria among each group (P>0.05). Conclusion High levels of steroid hormone do not affect expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase, indicating that SDF-1α/CXCR4 may not participate in the recruitment of CD56brightCD16-NK cells to endometria by steroid hormone.

Key words: endometrium, peri-implantation phase, chemokine, stromal cell-derived factor-1α, CXCR4, steroid hormone