›› 2011, Vol. 31 ›› Issue (12): 1715-.doi: 10.3969/j.issn.1674-8115.2011.12.012

• Original article (Basic research) • Previous Articles     Next Articles

Role of GDNF in regulation of expression of H19 and A-myb in spermatogonial stem cells

ZHA Wei-wei1, CAO Jing-ping2, ZHANG Di1, FENG Li-xin1, CHENG Jin-ke1   

  1. 1.Laboratory for Stem Cell Research, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;2.Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-12-28 Published:2012-01-04

Abstract:

Objective To investigate the regulation of expression of H19 and A-myb by glial cell line-derived neurotrophic factor (GDNF), and explore the molecular mechanism of self-renewal of spermatogonial stem cells regulated by GDNF. Methods Spermatogonial stem cells were isolated, identified and cultured from testes of 6-day-old mice with mixed enzyme digestion and differential plating procedure. Spermatogonial stem cells and spermatogonial stem cells pretreated with LY294002, inhibitor of phosphotylinosital 3 kinase/protein kinase B(PI3K/Akt), were stimulated with GDNF, and the expression of H19 and A-myb in spermatogonial stem cells was detected by RT-PCR. Results Spermatogonial stem cells were successfully isolated and cultured. The expression of H19 and A-myb was upregulated in spermatogonial stem cells after stimulation with GDNF, while there was no significant change in the expression of H19 and A-myb in spermatogonial stem cells after pretreatment with LY294002. Conclusion GDNF can upregulate the expression of H19 and A-myb via PI3K/AKT signal pathway in cultured mouse spermatogonial stem cells.

Key words: glial cell line-derived neurotrophic factor, spermatogonial stem cell, H19, A-myb, phosphotylinosital 3 kinase/protein kinase B