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    Original article (Basic research)
    Regulation of expression of osteoblast transcription factor SATB2 by BMP2 in osteoblastic differentiation of MSCs
    ZUO Chi-jian, LA Ting, ZHANG Ning, et al
    2011, 31 (12):  1661. 
    doi: 10.3969/j.issn.1674-8115.2011.12.001

    Abstract ( 1437 )   PDF (7066KB) ( 1069 )  

    Objective To investigate the role of bone morphogenetic protein 2 (BMP2) in the regulation of expression of osteoblast transcription factor SATB2 in osteoblastic differentiation of mesenchymal stem cells (MSCs). Methods Mesenchymal cell line C2C12 was stimulated by adenovirus-derived BMP2 (Adv-BMP2) for osteoblastic differentiation, and model of osteoblastic differentiation of C2C12 cells was established and verified. Real-Time PCR and Western blotting were employed to detect the expression of SATB2 mRNA and SATB2 protein respectively in osteoblastic differentiation treated by Adv-BMP2 for different time and by Adv-BMP2 of different concentrations. C2Cl2 cells treated with Adv-β-Gal were served as controls. Results After treatment by 150 pfu/cell Adv-BMP2 for 5 d, the expression of collagen type Ⅰ, bone sialoprotein and osteocalcin and the activity of alkaline phosphatase in C2C12 cells significantly increased, and the model of osteoblastic differentiation of C2Cl2 cells was established. The expression of SATB2 mRNA and SATB2 protein gradually increased with the progression of osteoblastic differentiation of C2Cl2 cells treated by 150 pfu/cell AdvBMP2, and the expression of SATB2 mRNA and SATB2 protein increased with the concentration increase of 0-225 pfu/cell Adv-BMP2. Conclusion BMP2 may influence osteoblastic differentiation of MSCs by regulating the expression of SATB2.

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    Role of ATP release in cyclic pressure-induced mucin excretion in airway epithelial cells
    SU Hui-hui, ZHOU Xiang-dong
    2011, 31 (12):  1667. 
    doi: 10.3969/j.issn.1674-8115.2011.12.002

    Abstract ( 1344 )   PDF (5498KB) ( 1186 )  

    Objective To investigate the role of adenosine triphosphate (ATP) release and intra-cellular calcium ion (Ca2+) on cyclic pressure-induced mucin (MUC) excretion in airway epithelial cells. Methods Human bronchial epithelial cell line 16HBE cultured in vitro were stimulated mechanically through tilting the culture dish, and the shear stress and compressive stress were provided by liquid surface tension, atmospheric pressure and liquid gravity. The airway epithelial cells were divided into control group, tilt group, tilt+Ca2+ chelant BAPTA-AM group, tilt+Ca2+ chelant EGTA group and tilt+P2Y receptor blocker RB-2 group. Cell survival rate was measured by MTT assay, expression of MUC5AC mRNA was detected by RT-PCR, expression of MUC5AC protein in culture supernatant was determined by ELISA, ATP release was examined by high performance liquid chromatography (HPLC), and intra-cellular Ca2+ concentration was observed by inverted fluorescence microscopy. Results Compared with control group, the expression of MUC5AC mRNA and protein in 16HBE cells and ATP content in the culture supernatant in tilt group were significantly higher (P<0.05). The expression of MUC5AC protein and ATP content in tilt+BAPTA-AM group and tilt+RB-2 group were significantly lower than those in tilt group (P<0.05). The expression of MUC5AC mRNA in tilt+EGTA group and tilt+RB-2 group was not significantly different from that in tilt group (P>0.05). Conclusion Cyclic mechanical stimulation induces ATP release and MUC5AC excretion in airway epithelial cells in a Ca2+ dependent manner.

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    Effect of local injection of endostatin on hypertrophic scar of rabbit ear
    WANG Xi-qiao, LIU Ying-kai, SONG Fei, et al
    2011, 31 (12):  1672. 
    doi: 10.3969/j.issn.1674-8115.2011.12.003

    Abstract ( 1269 )   PDF (8255KB) ( 937 )  

    Objective To investigate the effect of local injection of endostatin on hypertrophic scar of rabbit ear, and explore the mechanism. Methods Models of hypertrophic scar of rabbit ear were established. Four weeks after model establishment, scar tissues of right rabbit ears were injected with endostatin once a week for three weeks (experiment group, n=10), and scar tissues of left rabbit ears were injected with normal saline once a week for three weeks (control group,  n=10). Seven weeks after model establishment, scar tissues of rabbit ears in experiment group and control group were obtained, histomorphological changes were observed with HE staining, expression of microvessel marker CD34 was determined by immunohistochemistry, and apoptosis of fibroblasts was detected by TUNEL. Umbilical endothelial cells were cultured in vitro and seeded on Martrigel culture system with different concentrations of endostatin, and vessel tube formation of endothelial cells was observed. Results Compared with control group, the area of hypertrophic scar in experiment group significantly reduced, the number of fibroblasts in scar tissues decreased and collagen density became loose. The percent of cells with positive expression of CD34 in scar tissues in experiment group was significantly lower than that in control group [(2.21±0.39)% vs (6.11±1.32)%, P<0.05], while the percent of  apoptotic cells in experiment group was significantly higher than that in control group [(9.06±1.54)% vs (5.21±1.11)%, P<0.05]. In vitro observation revealed that more microvessel branches formed in Martrigel culture system without endostatin, and the number of microvessel tube formation gradually decreased with the increase of mass concentration of  endostatin. Conclusion Local injection of endostatin may inhibit hypertrophic scar development and promote scar regression, which may be correlated with the inhibition of microvessel tube formation.

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    Role of protein kinase C-&epsilon|in activation of endothelial nitric oxide synthase induced by propofol
    QI Ai-hua, WANG Li, CUI De-rong, et al
    2011, 31 (12):  1676. 
    doi: 10.3969/j.issn.1674-8115.2011.12.004

    Abstract ( 1288 )   PDF (6085KB) ( 1101 )  

    Objective To observe the effect of propofol on endothelial nitric oxide synthase (eNOS) activation, and explore the regulation role of protein kinase C-ε (PKC-ε) in drug-induced eNOS activation in human umbilical vein endothelial cells (HUVECs) model. Methods HUVECs of the third to fifth passages cultured in vitro were selected. Cells were treated with propofol (0 μmol/L, 1 μmol/L, 5 μmol/L, 10 μmol/L, 50 μmol/L and 100 μmol/L) for 1 h and 24 h respectively, and the concentration-effect relationship of propofol in activation of eNOS was analysed. Cells were exposed to propofol (5 μmol/L and 50 μmol/L) for 0 min, 5 min, 1 h, 6 h and 24 h, and time-effect relationship of propofol in activation of eNOS was explored. Then, cultured HUVECs were randomly divided into normal control group, PKC-ε pseudosubstrate+ propofol 5 μmol/L group, PKC-ε pseudosubstrate+ propofol 50 μmol/L group, single PKC-ε pseudosubstrate group, single propofol 50 μmol/L group and 10% long chain fat emulsion group. Cells in each group were treated for 1 h and 24 h respectively. The expression of PKC-ε, eNOS and P-Ser1177-eNOS protein was detected by Western blotting. Results Propofol increased the expression of P-Ser1177-eNOS protein in concentration- and time-dependent manners. When cells were treated for 24 h, the expression of P-Ser1177-eNOS protein in PKC-ε pseudosubstrate+ propofol 50 μmol/L group was significantly lower than that in single propofol 50 μmol/L group (P<0.05). Conclusion Propofol induces the activation of eNOS in concentration- and time-dependent manners, and PKC-ε positively mediates the activation of eNOS induced by propofol.

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    Fibrogenesis between dermal and adipose tissue-derived fibroblasts
    YUAN Bo, WANG Xi-qiao, QING Chun, et al
    2011, 31 (12):  1681. 
    doi: 10.3969/j.issn.1674-8115.2011.12.005

    Abstract ( 1543 )   PDF (7927KB) ( 918 )  

    Objective To investigate the fibrogenesis of dermal and adipose tissue-derived fibroblasts, and explore the mechanism. Methods Fibroblasts derived from dermal tissues and subcutaneous adipose tissues in the same pig were isolated and cultured. Inverted microscope and transmission electron microscope were used to observe the cell morphology and ultrastructure, and Real-Time PCR was employed to detect the relative expression of procollagen type ⅠmRNA, procollagen type Ⅲ mRNA, α-smooth muscle actin (α-SMA) mRNA, transforming growth factor β1 (TGF-β1) mRNA and insulin-like growth factor-1 (IGF-1) mRNA in cells. Results Compared with adipose tissue-derived fibroblasts, the cell body of dermal tissue-derived fibroblasts was larger, with expanded rough endoplasmic reticulum. The relative expression of precollagen type I mRNA and α-SMA mRNA in dermal tissue-derived fibroblasts was significantly higher than that in adipose tissue-derived fibroblasts (P<0.05), while the relative expression of precollagen type Ⅲ mRNA and IGF-1 mRNA in dermal tissue-derived fibroblasts was significantly lower than that in adipose tissue-derived fibroblasts (P<0.05). There was no significant difference in the relative expression of TGF-β1 mRNA between adipose tissue-derived fibroblasts and dermal tissuederived fibroblasts (P>0.05). Conclusion There are differences in cell morphology and function concerning with fibrogenesis between dermal tissuederived fibroblasts and adipose tissue-derived fibroblasts. The starting points of wound healing process after dermal and adipose tissue impairment are different between dermal tissue-derived fibroblasts and adipose tissue-derived fibroblasts.

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    Effects of 17-allylamino-17-demethoxygeldanamycin on apoptosis and expression of vascular endothelial growth factor in pheochromocytoma cells
    MA Gui, ZHU Yu, WU Yu-xuan, et al
    2011, 31 (12):  1687. 
    doi: 10.3969/j.issn.1674-8115.2011.12.006

    Abstract ( 1369 )   PDF (6891KB) ( 1009 )  

    Objective To investigate the effects of 17-allylamino-17-demethoxygeldanamycin (17-AAG), heat shock protein 90(HSP90) inhibitor, on the growth and expression of vascular endothelial growth factor (VEGF) in pheochromocytoma cell line PC12 in rats. Methods PC12 cells in experiment group were divided into experiment group 1 and experiment group 2. PC12 cells in experiment 1 were treated with different concentrations (0.005 μmol/L, 0.025 μmol/L, 0.05 μmol/L, 0.1 μmol/L, 0.25 μmol/L, 0.5 μmol/L, 1.0 μmol/L and 2.0 μmol/L) of 17-AAG culture fluid respectively, and those in experiment group 2 were treated with 150 μg/L VEGF culture fluid (VEGF group), 0.1 μmol/L 17-AAG culture fluid (17-AAG group) and 0.1 μmol/L17-AAG+150 μg/L VEGF culture fluid (17AAG+VEGF group) respectively. Besides, DMSO group (negative control group) and blank control group were also established. Cell survival rate was measured by MTT assay, cell morphology was observed by Wrights-Giemsa staining, cell apoptosis was detected by flow cytometry, and expression of VEGF-165 protein in cells was determined by Western blotting. Results 17-AAG significantly inhibited the growth of PC12 cells in time- and dose-dependent manners (P<0.05), with 50% inhibitory concentration (IC50) of 0.1 μmol/L. The apoptosis rates of PC12 cells after treatment with 0.1 μmol/L 17-AAG for 6 h, 12 h, 24 h and 48 h were significantly higher than that in blank control group (P<0.01). The expression of VEGF-165 protein gradually decreased with treatment of PC12 cells with 0.1 μmol/L 17-AAG for 6 h, 12 h, 24 h and 48 h, and the expression of VEGF-165 protein at each time point was significantly different from that in negative control group (P<0.05). Conclusion 17-AAG, HSP90 inhibitor, can inhibit the proliferation of PC12 cells, induce cell apoptosis and inhibit the expression of VEGF protein.

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    Role of interleukin-12 in regulation of expression of L-selectin on γδT cells
    WU Wen-juan, GENG Ying-hua, SHI Yu-rong, et al
    2011, 31 (12):  1692. 
    doi: 10.3969/j.issn.1674-8115.2011.12.007

    Abstract ( 1094 )   PDF (6081KB) ( 1131 )  

    Objective To investigate the role of interleukin (IL)-12 in regulation of the expression of L-selectin (CD62L) on γδT cells. Methods After induction and proliferation, human peripheral activated γδT cells were primarily and secondarily stimulated with Mtb-Ag. Cells were cultured with different cytokines (IFN-γ, IL-12+anti-IFN-γ, IL-12+anti-IL-12 and IL-12+IFN-γ) and different concentrations of IL-12 (0 ng/mL, 0.1 ng/mL and 1 ng/mL), respectively. Cell cultured for 0 d, 3 d, 5 d and 7 d were collected, and the expression of CD62L on γδT cells was detected by flow cytometry. Results After primary and secondary stimulation with Mtb-Ag, the expression of CD62L on γδT cells in IL-12 group, IL-12+anti-IFN-γ group and IL-12+ IFN-g group was significantly higher than that in blank control group (P<0.05), while the expression of CD62L on γδT cells in IFN-γ group and IL-12+anti-IL-12 group was not significantly different from that in blank control group (P>0.05). With the increase of concentrations of IL-12, the expression of CD62L on γδT cells increases. On the third day and fifth day after primary stimulation with Mtb-Ag, the expression of CD62L in 0.1 ng/mL IL-12 group and 1.0 ng/mL IL-12 group was significantly higher than that in negative control group (P<0.05), and the expression of CD62L in 1.0 ng/mL IL-12 group was significantly higher than that in 0.1 ng/mL IL-12 group (P<0.05). The expression of CD62L decreased after secondary stimulation with Mtb-Ag, while the decrease of expression of CD62L slowed down after treatment with IL-12, and the effect was more significant for 1.0 ng/mL IL-12 compared with 0.1 ng/mL IL-12 (P<0.05). Conclusion The expression of CD62L is dose-dependently regulated by IL-12 when γδT cells are activated.

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    Relationship between cell apoptosis and expression of hypoxia-induced factor-1alpha in retinal cells after ischemia-reperfusion injury in rats
    YUAN Hai-hong, ZHOU Wei, BAO Hui-ying, et al
    2011, 31 (12):  1697. 
    doi: 10.3969/j.issn.1674-8115.2011.12.008

    Abstract ( 1348 )   PDF (6922KB) ( 1077 )  

    Objective To investigate the changes of cell apoptosis and expression of hypoxia-induced factor-1α (HIF-1α) in retinal cells after ischemiareperfusion injury in rats, and explore the relationship between them. Methods Male SD rats were randomly divided into normal control group, 1 d after ischemia-reperfusion injury group, 3 d after ischemia-reperfusion injury group and 5 d after ischemia-reperfusion injury group (n=6). Retinal ischemia-reperfusion injury model was induced by perfusing saline into anterior chamber of eye to generate the intraocular pressure of 110 mmHg for 50 min. Retinal sections were prepared, the thickness of inner plexiform layer (IPL) and inner nuclear layer (INL) and the number of cells in ganglion cell layer (GCL) were measured, the retinal cell apoptosis was determined by TUNEL, the expression of HIF-1α in retinal cells was observed with immunohistochemical staining, and the expression of HIF-1α mRNA at different time points after injury was detected by RTPCR. Results Compared with normal control group, the thickness of IPL and INL was significantly lower (P<0.01), and the number of cells in GCL was significantly smaller in 1 d after ischemia-reperfusion injury group, 3 d after ischemia-reperfusion injury group and 5 d after ischemia-reperfusion injury group (P<0.05). Apoptosis of retinal cells occurred in GCL, and the apoptosis rates in 1 d after ischemia-reperfusion injury group, 3 d after ischemiareperfusion injury group and 5 d after ischemia-reperfusion injury group were significantly higher than that in normal control group (P<0.01). The rates of positive expression of HIF-1α in GCL of retina in 1 d after ischemia-reperfusion injury group, 3 d after ischemia-reperfusion injury group and 5 d after ischemia-reperfusion injury group were (47.88±14.71)%,(50.28±13.11)% and (43.09±10.04)% respectively, which were in line with the tendency of apoptosis of retinal cells (r=0.953). The expression of HIF-1α mRNA in 3 d after ischemia-reperfusion injury group was significantly higher than that in normal control group (P<0.05). Conclusion Both cell apoptosis and expression of HIF-1α in retinal cells increase after ischemiareperfusion injury in rats, and there is a positive correlation between them. The expression of HIF-1α may play an important role in retinal cell apoptosis.

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    Mechanism of losartan in treatment of insulin resistance in 3T3-L1 adipocytes
    LIU Xiao-li, PAN Yu, SHU Jin-lian, et al
    2011, 31 (12):  1702. 
    doi: 10.3969/j.issn.1674-8115.2011.12.009

    Abstract ( 1461 )   PDF (6856KB) ( 1125 )  

    Objective To investigate the main mechanism of losartan in treatment of insulin resistance in 3T3-L1 adipocytes. Methods The model of insulin resistance in 3T3-L1 adipocytes was induced by dexamethasone. Model control group (without treatment with any drug), losartan group (treatment with 1 μmol/L, 10 μmol/L and 100 μmol/L losartan for 48 h respectively) and wortmannin+losartan group were divided. Adipocytes in wortmannin+losartan group were pretreated with 100 nmol/L wortmannin, phosphatidylinositol 3-kinase (PI3K) inhibitor for 20 min, and were treated with 100 μmol/L losartan for 48 h. The size of adipocytes was observed, glucose oxidase method was employed to measure the glucose concentration in supernatant of culture fluid, and Western blotting was adopted to detect the expression of PI3K and insulin receptor substrate 1 (IRS-1) and level of IRS-1 serine phosphorylation in adipocytes. Results Compared with model control group, the size of adipocytes significantly reduced (P<0.01), the glucose concentration in supernatant of culture fluid significantly decreased (P<0.01), the expression of PI3K and IRS-1 significantly increased (P<0.01). The level of IRS-1 serine phosphorylation significantly decreased compared with model control group (P<0.01), but the effect could be blocked by wortmannin. Conclusion Losartan could significantly decrease the cell size and increase the consumption of glucose in 3T3-L1 adipocytes with insulin resistance, and the mechanism might be associated with PI3K pathway.

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    Effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats
    SHEN Li, YIN Jun, ZHOU Jun
    2011, 31 (12):  1707. 
    doi: 10.3969/j.issn.1674-8115.2011.12.010

    Abstract ( 1507 )   PDF (5040KB) ( 1274 )  

    Objective To investigate the effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats. Methods Twenty female Goto-Kakizaki (GK) rats with type 2 diabetes aged 6 weeks were randomized into resistant starch diet group (GK-RS group, n=10) and energy control diet group (GK-EC group, n=10), and another 10 age-matched female Wistar rats fed with energy control diet were served as blank control group (Wistar-EC group). Ten male offsprings were randomly selected in each group, and were raised till 8 weeks old on regular chow diet. Body weight and volume of food intake were recorded three time a week, pancreatic relative β cell density was measured by immunohistochemistry, fasting serum insulin concentration was detected by ELISA, concentrations of fasting blood glucose and 2 h postprandial blood glucose were determined by oral glucose tolerance test, and insulin sensitivity was evaluated by homeostasis model assessment of insulin resistance (HOMA-IR). Results Compared with GK-EC group, the concentration of fasting blood glucose significantly decreased (P<0.05), pancreatic insulin content significantly increased (P<0.05), and pancreatic relative β cell density tended to increase in GK-RS group (P=0.07). There was no significant difference in body weight, volume of food intake, HOMA-IR and concentrations of 2 h postprandial blood glucose and fasting serum insulin between GK-EC group and GK-RS group (P>0.05). Conclusion Dietary resistant starch has potential in improving glucose metabolism disorder in offsprings born to diabetic dams.

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    Expression of tumor necrosis factor-alpha and nuclear factor-κB and their correlation in hippocampus of rats with status epilepticus
    SONG Sha-sha, GENG Zhi, GAO Hua
    2011, 31 (12):  1711. 
    doi: 10.3969/j.issn.1674-8115.2011.12.011

    Abstract ( 1347 )   PDF (4866KB) ( 1070 )  

    Objective To investigate the expression of tumor necrosis factor-α (TNF-α) and nuclear factor (NF-κB) in hippocampus of rats with status epilepticus (SE). Methods Twenty Wistar rats were intraperitoneally injected with 10% pilocarpine (300 mg/kg)(SE group),and injection was ceased 60 min after SE onset. Besides, six rats treated with same amount of normal saline were served as control group. Hippocampus tissues of rats were obtained 24 h after treatment, the number of neurons in CA3 area of hippocampus was observed with HE staining, the expression of NF-κB and TNF-α in hippocampus tissues was determined by Western blotting and ELISA respectively. Pearson correlation analysis was adopted to explore the correlation between the expression of TNF-α and that of NF-κB. Results The number of neurons in CA3 area of hippocampus in SE group was much smaller than that in control group (53.26±2.67 vs 103.22±0.83, P<0.001). The expression of TNF-α and NF-κB in hippocampus tissues in SE group was significantly higher than that in control group (P<0.001, P<0.05), and there was a positive correlation between the expression of TNF-α and that of NF-κB in SE group (r=0.457,P=0.02). Conclusion The expression of TNF-α and NF-κB in hippocampus tissues of rats increases 24 h after SE, and there is a positive correlation between the expression of TNF-α and that of NF-κB.

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    Role of GDNF in regulation of expression of H19 and A-myb in spermatogonial stem cells
    ZHA Wei-wei, CAO Jing-ping, ZHANG Di, et al
    2011, 31 (12):  1715. 
    doi: 10.3969/j.issn.1674-8115.2011.12.012

    Abstract ( 1347 )   PDF (6310KB) ( 1096 )  

    Objective To investigate the regulation of expression of H19 and A-myb by glial cell line-derived neurotrophic factor (GDNF), and explore the molecular mechanism of self-renewal of spermatogonial stem cells regulated by GDNF. Methods Spermatogonial stem cells were isolated, identified and cultured from testes of 6-day-old mice with mixed enzyme digestion and differential plating procedure. Spermatogonial stem cells and spermatogonial stem cells pretreated with LY294002, inhibitor of phosphotylinosital 3 kinase/protein kinase B(PI3K/Akt), were stimulated with GDNF, and the expression of H19 and A-myb in spermatogonial stem cells was detected by RT-PCR. Results Spermatogonial stem cells were successfully isolated and cultured. The expression of H19 and A-myb was upregulated in spermatogonial stem cells after stimulation with GDNF, while there was no significant change in the expression of H19 and A-myb in spermatogonial stem cells after pretreatment with LY294002. Conclusion GDNF can upregulate the expression of H19 and A-myb via PI3K/AKT signal pathway in cultured mouse spermatogonial stem cells.

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    Successive composite tissue allotransplantation on lung allotransplanted rat model
    WANG Shou-bao, Jui-Chih Chang, LIU Fei, et al
    2011, 31 (12):  1719. 
    doi: 10.3969/j.issn.1674-8115.2011.12.013

    Abstract ( 1171 )   PDF (8288KB) ( 1000 )  

    Objective To investigate the immunosuppression effect of successive composite tissue allotransplantation(SCTA)after previous parenchymatous organ transplantation in rats. Methods Left lungs from WKY rats were orthotopically transplanted into F344 rats, and cuff technique was employed for anastomoses of the left pulmonary vein, left pulmonary artery and main bronchus. F344 rats were subcutaneously injected with cyclosporine A (5 mg/kg) once every two days for 10 d after operation. Transplanted pulmonary function was assessed with respiratory rate. On the eleventh day after lung transplantation, 3 cm×3 cm abdominal musculocutaneous flaps harvested from Brown Norway rats were transplanted to F344 rats, and the abdominal flap vessels were connected to the femoral vessels. No further immunosuppression was administered following flap transplantion. Ten days after composite tissue allotransplantation (CTA), transplanted flaps and lung tissues were obtained for histopathological examinations, and rejection reaction was observed. Results The successful rates of lung transplantation, CTA and SCTA were 90%, 100% and 90% respectively. The respiratory rates increased from (61±10) times/min on day 10 after transplantation (immunosuppression for 10 d) to (75±15) times/min on day 20 after transplantation (immunosuppression withdrawl for 10 d). The allotransplanted lung exhibited typical signs of acute rejection, including both perivascular and diffuse lymphocytic infiltration. There was no acute rejection in abdominal flap autograft by histopathological observation, and the allotransplanted abdominal flaps had typical perivascular and diffuse lymphocytic infiltration after CTA. Conclusion SCTA rat model may facilitate the research of secondary CTA following primary parenchymatous organ transplantation and can be useful in developing future immunotherapeutic strategies.

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    Original article (Clinical research)
    Event-based prospective memory during acute phase schizophrenia
    ZHUO Kai-ming, YANG Zhi-liang, SONG Zhen-hua, et al
    2011, 31 (12):  1724. 
    doi: 10.3969/j.issn.1674-8115.2011.12.014

    Abstract ( 1332 )   PDF (6552KB) ( 1116 )  

    Objective To investigate the event-based prospective memory deficit in patients with acute and drug-free schizophrenia. Methods Forty-nine healthy controls (healthy control group) and 51 patients with acute and drug-free schizophrenia (schizophrenia group) were enrolled, and the latter were further divided into first-episode schizophrenia subgroup (n=23) and chronic schizophrenia subgroup (n=28). Prospective memory was assessed with event-based prospective memory pattern, retrospective memory was determined with recognition test, visuo-spatial working memory was examined with spatial span test, and the severity of psychotic symptoms was evaluated with Positive and Negative Symptom Scale (PANSS). Results The prospective memory, retrospective memory and visuo-spatial working memory in schizophrenia group were significantly lower than those in healthy control group (P=0.008, P=0.016 and P=0.001 respectively). After controlling for retrospective memory and visuo-spatial working memory, the prospective memory in schizophrenia group was still lower than that in healthy control group (P<0.05). In schizophrenia group, prospective memory was positively correlated with education (r=0.405,P<0.05), and was negatively correlated with age, disease duration, PANSS total score and negative symptom score (r=-0.41, r=-0.5, r=-0.345 and r=-0.486 respectively, P<0.05 for all). There was no significant difference in prospective memory, retrospective memory and visuo-spatial working memory between first-episode schizophrenia subgroup and chronic schizophrenia subgroup (P<0.05 for all). Conclusion There exists significant prospective memory deficit during acute phase schizophrenia, and the prospective memory deficit in first-episode patients is similar to that of the chronic patients. The prospective memory deficit in patients with schizophrenia is a kind of primary cognitive deficit.

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    Efficacy and safety of budesonide suspension fluid combined with tulobuterol tape in treatment of recurrent wheezing in infants and young children
    ZHU Ya-ju, LIN Qian, HUA Li, et al
    2011, 31 (12):  1729. 
    doi: 10.3969/j.issn.1674-8115.2011.12.015

    Abstract ( 1466 )   PDF (4079KB) ( 1197 )  

    Objective To evaluate the efficacy and safety of budesonide suspension fluid inhalation combined with tulobuterol tape in treatment of recurrent wheezing in infants and young children. Methods Sixty-two infants and young children with recurrent wheezing were randomly divided into  treatment group (n=31) and control group (n=31). When wheezing was relieved, patients in  treatment  group were treated with budesonide suspension fluid inhalation combined with tulobuterol tape for 12 weeks, while those in control group were treated with  budesonide suspension fluid inhalation only. Patients were followed up during the 2nd, 4th, 6th, 8th, 10th and 12th week, and the frequencies of cough and respiratory tract infection and the condition of combination use of drugs were analysed. Results Thirty patients in  treatment group and 29 patients in control group completed the treatment and follow up. The scoring of cough, frequency of wheezing, scoring of wheezing and scoring of combination use of drugs in  treatment group were significantly lower than those in control group (P<0.01), and the frequency of respiratory tract infection in  treatment group was significantly lower than that in control group (P<0.05). Conclusion Budesonide suspension fluid inhalation combined with tulobuterol tape is more effective and safe than budesonide suspension fluid inhalation only in treatment of recurrent wheezing in infants and young children.

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    Application of intraoperative somatosensory evoked potential combined with microvascular Doppler sonography in intracranial aneurysm clipping surgery
    FEI Zhi-min, ZHANG Jue, SHU Guo-wei, et al
    2011, 31 (12):  1733. 
    doi: 10.3969/j.issn.1674-8115.2011.12.016

    Abstract ( 1309 )   PDF (5095KB) ( 1133 )  

    Objective To evaluate the application of intraoperative somatosensory evoked potential (SEP)combined with microvascular Doppler sonography (MDS) in intracranial aneurysm clipping surgery. Methods Fifty-three patients with a total of 57 intracranial aneurysms undergoing intraoperative intracranial aneurysm clipping surgery were evaluated with intraoperative SEP and MDS, and the clips were repositioned according to the findings of MDS and SEP. Patients were reexamined with digital subtraction angiography (DSA) after operation,and were evaluated with Glasgow outcome scale (GOS) one month after operation. Results There was no persistent change of SEP in all patients during surgical procedure.Aneurysms were distinguished from parent arteries in 9 (17.0%) patients by MDS. Persistent blood flow through the aneurysmal sac was revealed in 3 (5.3%) aneurysms after clipping, and the clips were repositioned. Parent artery stenosis after clipping was indicated by MDS in 6 (11.3%) cases, and the clips were repositioned. DSA revealed that there was no aneurysmal residue and stenosis of parent and adjacent vessels in 50 patients, which was in line with the findings by SEP and MDS during operation. Patients were followed up 1 month after operation, two patients died, and the other patients had 5 points (41 patients), 4 points (8 patients), 3 points (1 patient) and 2 points (1 patient) of GOS respectively. Conclusion Application of intraoperative SEP combined with MDS is a noninvasive, convenient and effective technique in intracranial aneurysm clipping surgery, which may help to improve the surgical outcomes.

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    Value of EBUS-TBNA cytopathological detection in diagnosis of lung cancer and sarcoidosis
    HAN Fei, ZHANG Jian, SUN Jia-yuan, et al
    2011, 31 (12):  1737. 
    doi: 10.3969/j.issn.1674-8115.2011.12.017

    Abstract ( 1946 )   PDF (5741KB) ( 1424 )  

    Objective To investigate the value of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) cytopathological detection in diagnosis of lung cancer and sarcoidosis, and analyse the clinicopathological characteristics. Methods One hundred and sixty cases undergoing cytopathological detection were collected, and the value of cytopathological detection in the diagnosis of lung cancer and sarcoidosis was analysed based on the criteria of histologiopathology combined with clinical manifestations. Results The diagnosis of 150 cases was confirmed by histopathological examinations, including 108 cases of lung cancer, 23 cases of sarcoidosis, 4 cases of tuberculosis and 15 cases of the other diseases. Ninety-five cases of lung cancer were detected by EBUS-TBNA cytopathological detection, with the positive diagnostic rate of 88.0% (95/108) and negative diagnostic rate of 12.0% (13/108). The sensitivity, specificity, positive predictive value and negative predictive value of EBUS-TBNA cytopathological detection in diagnosis of lung cancer were 88.0%, 100%, 100% and 76.4% respectively. EBUS-TBNA cytopathological detection revealed 15 cases of non-caseous granuloma lesion among 23 cases of sarcoidosis, and 1 case of caseous granuloma in 4 cases of tuberculosis. Conclusion EBUS-TBNA cytopathology detection has high sensitivity and specificity in diagnosis of lung cancer, and may play a role in the diagnosis of sarcoidosis and tuberculosis.

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    Early and midterm outcomes of acute lower extremity deep venous thrombosis treated by catheter directed thrombolysis
    YIN Min-yi, JIANG Mi-er, LI Wei-min, et al
    2011, 31 (12):  1741. 
    doi: 10.3969/j.issn.1674-8115.2011.12.018

    Abstract ( 1197 )   PDF (5480KB) ( 1797 )  

    Objective To investigate the early and midterm outcomes of acute lower extremity deep venous thrombosis (DVT) treated by catheter directed thrombolysis (CDT). Methods Thirty-three patients with DVT underwent CDT treatment. The early outcome after CDT treatment was assessed by the rate of patency improvement and differences in circumference between impaired thigh and healthy thigh and between impaired leg and healthy leg after CDT treatment, and the midterm outcome was evaluated with percents of normal first valve in superficial femoral vein and venous clinical severity score (VCSS). Results The initial technical success rate of CDT treatment was 100%, with 5 cases of minor bleeding complications. The mean duration of CDT treatment was (3.93±1.14) d. The mean rate of patency improvement after treatment was (69.25±18.93)%. The differences in circumference between impaired thigh and healthy thigh and between impaired leg and healthy leg after treatment were significantly smaller than those before treatment(P<0.01, P<0.05). Twenty-nine patients were successfully followed up for 6 to 27 months. Three months and 6 months after treatment, the percents of normal first valve in superficial femoral vein in patients with thrombolysis grade Ⅱ and Ⅲ were significantly higher than that in patients with thrombolysis grade Ⅰ(P<0.05, P<0.01), and VCSS in patients with thrombolysis grade Ⅱ and Ⅲ were significantly lower than that in patients with thrombolysis grade Ⅰ(P<0.01). Conclusion Favorable early and midterm outcomes with less severe complications may be yielded in patients with acute lower extremity DVT treated by CDT.

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    Effects of budesonide suspension inhalation on relieving postoperative throat complications after tracheal intubation under general anesthesia
    CHEN Yan-qing, WANG Jia-dong, XU Ya-nan, et al
    2011, 31 (12):  1746. 
    doi: 10.3969/j.issn.1674-8115.2011.12.019

    Abstract ( 1617 )   PDF (4334KB) ( 1122 )  

    Objective To investigate the effects of budesonide suspension inhalation on relieving postoperative throat complications after tracheal intubation under general anesthesia. Methods One hundred and ten patients with benign thyroid tumors undergoing tracheal intubation under general anesthesia were randomly divided into group A (n=40, 2 mg budesonide suspension inhalation 1 h before operation and 6 h, 24 h and 48 h after operation), group B (n=40, 2 mg budesonide suspension inhalation 6 h, 24 h and 48 h after operation) and group C (n=30, inhalation of normal saline 6 h, 24 h and 48 h after operation). The conditions of throat were observed in each group after operation, and the effectiveness of different methods of suspension inhalation in signs and symptoms after tracheal intubation was evaluated in each group. Results The incidences of sore throat, cough and hoarseness after operation in all patients were 99%, 13.6% and 53.6% respectively. The scoring of sore throat at each time point after operation and hoarseness 1 h, 6 h and 24 h after operation in group A was better than that in group B (P<0.05). There were significant differences in the scoring of sore throat and hoarseness 48 h after operation between group B and control group (P<0.05), while there was no significant difference in the scoring of sore throat and hoarseness at the other time points between two groups (P>0.05). There was no significant difference in the scoring of cough among three groups (P>0.05). The scoring of mucosa reaction of throat 1 h, 6 h and 24 h after operation in group A was better than that in group B and control group, and the scoring of mucosa reaction of throat 6 h, 24 h and 48 h after operation in group B was better than that in control group (P<0.05). Conclusion Inhalation of budesonide suspension is effective in reducing the throat injury caused by tracheal intubation under general anesthesia, and preoperative administration may have preventive function to some degree.

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    Relationship between plasma glucose levels and macrosomia in women with abnormal glucose metabolism during pregnancy
    LI Hua-ping, SUN Ping-ping
    2011, 31 (12):  1750. 
    doi: 10.3969/j.issn.1674-8115.2011.12.020

    Abstract ( 1405 )   PDF (5864KB) ( 1264 )  

    Objective To investigate the relationship between plasma glucose levels in oral glucose-tolerance test (OGTT) and macrosomia in women with abnormal glucose metabolism during pregnancy. Methods The clinical data of 125 pregnant women with gestational diabetes mellitus (GDM) and 21 women with gestational impaired glucose tolerance (GIGT) during pregnancy were retrospectively analysed. Macrosomia group (n=36) and nonmacrosomia group (n=110) were divided according to the delivery outcomes. T-test, chi-square test and multivariate Logistic regression analysis were employed to analyse the relationship between plasma glucose levels in OGTT and macrosomia in women with abnormal glucose metabolism during pregnancy. Results ①There were significant differences in fasting plasma glucose (FPG) and 1 h plasma glucose in OGTT between two groups (P<0.05), while there was no significant difference in 50 g glucose challenge test (GCT) value, 2 h plasma glucose in OGTT and 3 h plasma glucose in OGTT between two groups (P>0.05). ②Pregnant women with FPG<5.3 mmol/L and odd ratios (OR) <1 had a lower risk for macrosomia, while those with FPG ≥5.3 mmol/L and OR >1 had a higher risk for macrosomia. ③OR increased with weight gain during pregnancy, and the risk for macrosomia increased with the weight gain. Pregnant women with FPG>5.1 mmol/L and weight gain>16-18 kg had a significant higher risk for macrosomia. Conclusion For women with abnormal glucose metabolism during pregnancy, high level of FPG is a major risk factor for macrosomia. Poor blood glucose control in pregnancy may lead to macrosomia and related complications.

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    Surveillance of drug resistance of Staphylococcus aureus
    WU Jing, YING Chun-mei, WANG Ya-ping, et al
    2011, 31 (12):  1754. 
    doi: 10.3969/j.issn.1674-8115.2011.12.021

    Abstract ( 1211 )   PDF (4559KB) ( 1390 )  

    Objective To analyse the clinical distribution of Staphylococcus aureus and detection rate of methicillin resistant Staphylococcus aureus (MRSA), and compare the resistance of MRSA and methicillin sensitive Staphylococcus aureus(MSSA) to antibiotics. Methods Eighty isolates of Staphylococcus aureus were collected from clinical specimens from July to September in 2010. Cefoxitin disk diffusion method and polymerase chain reaction (PCR) were carried out to screen isolates of MRSA. Agar dilution was conducted to determine the minimum inhibitory concentrations (MICs) of 11 antibiotics for MRSA and MSSA, and the differences in drug resistance were compared. Results Forty-six (57.50%) isolates of MRSA were detected from 80 isolates of Staphylococcus aureus. Forty-five (56.25%) isolates of MRSA were screened after detection of mecA gene by PCR. Department of Neurosurgery (17/45, 37.78%), Department of General Surgery (7/45, 15.56%) and Emergency Observation Unit (5/45, 11.11%) were the sections with highest detection rates of MRSA. All the 80 isolates of Staphylococcus aureus were sensitive to linezolid, vancomycin and teicoplanin. However, there were significant differences in drug resistance to the other 8 antibiotics between MRSA and MSSA (P<0.05). Conclusion The detection rate and drug resistance rate of MRSA are high, which should arouse more attention from clinical departments and microbiology laboratory, so as to avoid largescale epidemics caused by nosocomial infection.

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    Application of bone scintigraphy in therapy response monitoring and prognosis prediction in patients with bone metastasis from lung cancer and prostate cancer
    PAN Yi-fan, LIU Jian-jun, HUANG Gang, et al
    2011, 31 (12):  1758. 
    doi: 10.3969/j.issn.1674-8115.2011.12.022

    Abstract ( 1679 )   PDF (6571KB) ( 1121 )  

    Objective To investigate the application of bone scintigraphy in therapy response monitoring and prognosis prediction in patients with bone metastasis from lung cancer and prostate cancer. Methods Whole-body bone scintigraphy was performed in 40 patients with lung cancer and 31 patients with prostate cancer one month before systematic therapy and no less than 3 months after treatment. The changes of bone metastasis lesions were observed before and after treatment, and the correlation of bone metastasis with therapy response was explored. Survival rates were calculated by Kaplan-Meier method, and prognostic factors for survival were analysed by Log-rank test and Cox regression model. Results The therapeutic effect of bone metastasis in clinical therapy responders in primary tumors (59.68%, 37/62) was significantly better than that in clinical therapy non-responders in primary tumors (40.32%, 25/62)(P<0.05). The 1-year and 2-year cumulative survival rates of patients with bone metastasis from lung cancer were 54.5% and 22.6% respectively, and those of patients with bone metastasis from prostate cancer were 87.3% and 72.3% respectively. Both univariate analysis and multivariate analysis indicated that tumor type and duration of bone metastasis were related to survival rates of patients with bone metastasis from lung cancer and prostate cancer (P<0.05). Cox regression analysis revealed that pathological type, extent of disease before treatment and duration of bone metastasis were prognostic factors in patients with bone metastasis from lung cancer, and duration of bone metastasis was the prognostic factor in patients with bone metastasis from prostate cancer. Conclusion Whole-body bone scintigraphy provides more abundant and exact information in therapy response monitoring and prognosis prediction in patients with bone metastasis from lung cancer and prostate cancer.

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    Original article (Public health administration)
    Effects of new rural cooperative medical health policy on self-perceived health of rural residents
    FAN Tao, CAO Qian, JIANG Lu-lu, et al
    2011, 31 (12):  1763. 
    doi: 10.3969/j.issn.1674-8115.2011.12.023

    Abstract ( 1198 )   PDF (4290KB) ( 1261 )  

    Objective To analyse the effects of new rural cooperative medical policy on the health of rural residents, and quantitatively evaluate the implementation of the policy. Methods Data on rural residents participating in new rural cooperative medical policy in 2000 and 2006 were evaluated with 2×2 square analysis and Probit model based on difference in differences method, and the implementation of policy was analysed. Results The number of females participating in new rural cooperative medical policy was larger than that of males, and the mean age of residents participating in the policy (45.79 years old) was lower than that of residents without participation (46.84 years old). The overall self-perceived health value in 2006 was lower than that in 2000. The policy had positive effect on self-perceived health value, with difference in differences of 0.040 3. Gender, age, drinking and medical insurance participation had influence on self-perceived health value (P<0.05). Conclusion The new rural cooperative medical policy has a positive effect on self-perceived health, but the effect may be hindered by potential moral risk and self-ignorance. Health education should be enhanced during the implementation of the policy.

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    Establishment of competency model of senior managerial talent for health service
    CAI Yu-yang, ZHAO Qing, JIANG Xue-qin, et al
    2011, 31 (12):  1767. 
    doi: 10.3969/j.issn.1674-8115.2011.12.024

    Abstract ( 1241 )   PDF (6183KB) ( 1095 )  

    Objective To establish competency model of senior managerial talent for health service in Shanghai. Methods Expert interview was conducted to screen characteristic clusters from McClelland's Competency Dictionary, and the questionnaire for survey was obtained. Two hundred and twenty managerial talents for health service of Shanghai were surveyed with questionnaires, and the characteristic competency data were obtained. Results One hundred and seventy-eight questionnaires were recovered, with the recovery rate of 80.9%. The competency model of senior managerial talent for health service included 8 characteristic clusters, 26 characteristic items and 26 typical behavior descriptions. The characteristic clusters were personal character, success orientation, social role, self concept, knowledge management, communication and coordination, cost management and business management. Conclusion The competency model of senior managerial talent for health service provides clear description and comprehensive indicator system in characteristic clusters and characteristic items, while there is still room for improvement in practical application in health service.

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    Analysis of awareness and demand on special medical services of patients in Shanghai
    ZHANG Jing, HE Li-ming, YAN Rong, et al
    2011, 31 (12):  1771. 
    doi: 10.3969/j.issn.1674-8115.2011.12.025

    Abstract ( 1495 )   PDF (4966KB) ( 954 )  

    Objective To investigate the awareness and demand on special medical services of patients in Shanghai, and put forward suggestions on the development of special medical services. Methods Five hundred and one patients randomly selected from 5 public hospitals in Shanghai were subjected to questionnaire survey, and patients were divided into special medical care group (n=234) and ordinary medical care group (n=267) according to the experience of special medical services. The survey included five aspects of basic conditions, experience and attitude towards special medical services, demand on special medical services, payment of special medical services and advice for special medical services. Results Patients older than 30 years accounted for 76.5% in special medical care group, and 96.6% of patients in special medical care group believed that special medical services should be provided in public hospitals, which was significantly higher than that in ordinary medical care group (53.9%) (P<0.000 1). It was considered the price of special medical services was unreasonable by 42.7% of patients in special medical care group. Among all the patients, 37.0% thought high-quality medical services (services provided by medical experts) was essential for special medical services, and expert outpatient services were preferred by 52.0% of patients. Conclusion Patients hold positive attitudes towards special medical services in public hospitals, and the scope and content of special medical services are to be clarified.

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    Review
    Research progress of chemokine receptor-5 related cardiac allograft vasculopathy
    JIANG Zhao-lei, HE Yi, MEI Ju
    2011, 31 (12):  1775. 
    doi: 10.3969/j.issn.1674-8115.2011.12.026

    Abstract ( 1471 )   PDF (3496KB) ( 880 )  

    Cardiac allograft vasculopathy (CAV) continues to limit the long-term success of cardiac transplantation and has become a leading cause for death and retransplantation in patients who survive beyond the first year after heart transplantation. At present, it is considered that both immunologic and nonimmunologic risk factors contribute to the development of CAV by causing endothelial dysfunction and injury, eventually leading to progressive intimal thicking and ischemic failure of the cardiac allograft. Chemokines and their receptors may participate in regulating the local immune response of the graft. Among them, chemokine receptor-5 (CCR5) may play an important role in the pathogenesis of CAV, but the mechanism is unclear. So far, the therapy for CAV has been relatively limited, but there have been some new findings with the research progress of CCR5, which provides a new direction for the treatment and prevention of CAV.

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    Influence of cyclophilin A and its receptor CD147 on atherosclerosis
    ZHANG Tian-tian, ZHANG Jun-feng
    2011, 31 (12):  1778. 
    doi: 10.3969/j.issn.1674-8115.2011.12.027

    Abstract ( 1386 )   PDF (4467KB) ( 1326 )  

    Cyclophilin A (CyPA) participates in the whole development process of atherosclerosis through its receptor molecule CD147. During the process, cell injury, apoptosis and activation were caused, and dysfunction of endothelial cells emerged. The migration of leukocytes such as neutrophils, eosinophils, monocytes/macrophages and T lymphocytes to atherosclerotic lesions occurred, various inflammatory cytokines were produced, and inflammation was aggravated. The secretion of matrix metalloproteinases from macrophages was induced, and the instability of atheromatous plaques was increased. The formation of foam cells induced by macrophages' internalization of lipids was mediated, and the formation of fatty streaks was accelerated. Besides, the incrassation and reconstruction of vascular walls mediated by the proliferation of macrophages and vascular smooth muscle cells were promoted. Therefore, research on the roles of CyPA and CD147 may provide new directions for atherosclerosis intervention.

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    Mechanism of epithelial-mesenchymal transition in epithelial ovarian cancer
    ZHONG Yi-cun, LI Wei-ping, DI Wen
    2011, 31 (12):  1782. 
    doi: 10.3969/j.issn.1674-8115.2011.12.028

    Abstract ( 1414 )   PDF (4215KB) ( 1142 )  

    Epithelial ovarian cancer (EOC) is the leading cause for death among gynecologic malignancies, which is closely related to its biological behaviors such as high potential of invasion and metastasis as well as chemoresistance and recurrence.The above features of EOC are closely associated with epithelial-mesenchymal transition (EMT). EMT means that epithelial cells lose the polarity, shape and arrangement, regains stromal cells' cytoskeleton and morphology, which significantly changes the cell migration phenotype. Thus, profound research and exploration of the mechanism of EMT in EOC may help to find new ways to treat EOC, and the blockage or interference with EMT in EOC may become a new target. The mechanism of EMT in EOC is reviewed in this paper.

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    Research progress of microRNA and renal fibrosis
    LIU Jian, WANG Wei-ming
    2011, 31 (12):  1786. 
    doi: 10.3969/j.issn.1674-8115.2011.12.029

    Abstract ( 1322 )   PDF (4095KB) ( 1180 )  

    microRNA (miRNA), consisted of about 22 nucleotides, are noncoding small RNA. They can impact the target RNA, thus regulating the physiological and pathological processes and possessing an significant action in cell proliferation, apoptosis, and differentiation. It has been reported that miRNA, modulating the deposition of collagen, expression of fibronectin, epithelial-to-mesenchymal transition and effect of transforming growth factor-β in kidney, are involved in the mechanism of renal fibrosis.

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    Brief original article
    Analysis of electromyographic activity and chewing efficiency of zero-degree occlusal dentures after restoration
    QIAN Hai-xin, YANG Dan-ling
    2011, 31 (12):  1790. 
    doi: 10.3969/j.issn.1674-8115.2011.12.030

    Abstract ( 1476 )   PDF (2870KB) ( 924 )  

    Objective To investigate the chewing function of zero-degree occlusal dentures after restoration. Methods Twenty patients with complete dentures were divided into zero-degree occlusal dentures group and anatomic occlusal dentures group, with 10 patients in each group. Bilateral temporalis and masseter activities were examined by electromyography soon after denture placement and one month after denture placement, and the chewing efficiencies of these two dentures were compared. Results There was no significant difference in peak electromyographic values between zerodegree occlusal dentures group and anatomic occlusal dentures group soon after denture placement and one month after denture placement (P>0.05). The peak electromyographic value of tapping teeth movement one month after denture placement was significantly higher than that soon after denture placement in anatomic occlusal dentures group (P<0.05), while there was no significant difference in zero-degree occlusal dentures group (P>0.05). The resting phase of electromyographic activity one month after denture placement was significantly shorter than that soon after denture placement in zero-degree occlusal dentures group (P<0.05), and was significantly shorter than that one month after denture placement in anatomic occlusal dentures group (P<0.05). There was no significant difference in the chewing efficiencies one month after denture placement between two groups (P>0.05). Conclusion Zero-degree occlusal dentures can produce similar masticatory function as anatomic occlusal dentures and better adaptiveness for patients with unfavorable alveolar ridge.

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    Improvement of survival in patients with newly diagnosed multiple myeloma treated by Bortezomib based chemotherapy
    ZHAO Bing-bing, WANG Yan, MI Jian-qing, et al
    2011, 31 (12):  1793. 
    doi: 10.3969/j.issn.1674-8115.2011.12.031

    Abstract ( 2126 )   PDF (4820KB) ( 1048 )  

    Objective To evaluate the curative effect of Bortezomib based chemotherapy in treatment of patients with newly diagnosed multiple myeloma, and explore the prognositic factors. Methods Thirty-eight patients with newly diagnosed multiple myeloma received Bortezomib based chemotherapy, and the drugs used in chemotherapy included Melphalan+Prednisone, Dexamethasone, Dexmethasone+Cyclophosphamide or Dexamethasone+Adriamycin. Each course of treatment lasted for 3 to 4 weeks, and intravenous injection of 1.3 mg/m2 Bortezomib was performed on the 1st, 4th, 8th and 11th day of each course. Patients were managed with 2 to 10 courses. Response to Bortezommib was assessed according to the criteria of European Group for Blood and Marrow Transplantation, and adverse events were evaluated according to the criteria of National Cancer Institute Common Terminology Criteria for Adverse Events Version 3.0. The prognosis factors were analysed by Log-rank test and COX regression model. Results The median duration of follow-up was 19 months. The overall response rate (complete remission+very good partial remission+partial remission) was 86.84%. The median progression-free survival was 19.47 months, and the median overall survival was 39.17 months. Univariate analysis and multivariate analysis revealed that complete remission and very good partial remission after treatment were prognostic factors, while M protein typing, age, Durie-salmon staging, high serum creatinine, low hemoglobin, low albumin, high β2 microglobulin and high lactate dehydrogenase were not related to prognosis. The major adverse events during treatment were gastrointestinal sympotoms, leukocytopenia, thrombocytopenia, peripheral neuropathy and fatigue. Conclusion Bortezomib based chemotherapy is well tolerated and effective in treatment of patients with newly diagnosed multiple myeloma, which overcomes the poor prognosis conferred by traditional prognosis factors.

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