›› 2011, Vol. 31 ›› Issue (4): 397-.doi: 10.3969/j.issn.1674-8115.2011.04.003

• Original article (Basic research) • Previous Articles     Next Articles

Effects of mechanical stretching on expression of mucin5AC in airway epithelial cells and its mechanism of signaling pathway

ZHONG Tian1, Juliy M. Perelman2, Victor P.Kolosov2, ZHOU Xiang-dong1   

  1. 1.Department of Respiratory Medicine, The Second Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400010, China；2.Far Eastern Scientific Center of Physiology and Pathology of Respiration, |Russian Academy of Medical Sciences, Blagoveschensk 675000, Russia
  • Online:2011-04-28 Published:2011-04-28
  • Supported by:

    National Natural Science Foundation of China, 81070031； Cooperation Project of China and Russia of National Natural Science Foundation of China, 81011120108

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Abstract:

Objective To investigate the effects of mechanical stretching on the expression of mucin(MUC)5AC in airway epithelial cells, and explore its mechanism of signaling pathway. Methods Mini-type multi-fuctional bio-impact machine was used to construct the model of mechanical stretching of airway epithelial cells. Three inhibitors of mitogen-activated protein kinase (MAPK) signaling pathway (JNK inhibitor, SP600125; p38 protein kinase inhibitor, SB203580; and ERK1/2 inhibitor, U0126) were administered at the concentration of 30 μmol/L, respectively. Besides, control group without mechanical stretching and single mechanical stretching group were established. Free calcium concentrations in cells before and after mechanical stretching were determined by flow cytometry and observed by laser confocal microscopy. The expression of MUC5AC mRNA and protein was detected by RT-PCR and ELISA, respectively. Results Mechanical stretching increased the concentration of free calcium and expression of MUC5AC mRNA and protein in cells （P<0.01). U0126 and SB203580 decreased the expression of MUC5AC mRNA and protein (P<0.01). Conclusion Mechanical ventilation could increase the expression of MUC5AC in airway endothelial cells, the mechanism of which may be concerned with the increase of calcium concentration and ERK1/2 and p38 MAPK signaling pathway in cells.

Key words: mechanical stretching, free calcium ion, mitogen-activated protein kinase, mucin5AC