›› 2012, Vol. 32 ›› Issue (3): 247-.doi: 10.3969/j.issn.1674-8115.2012.03.003

• Original article (Basic research) • Previous Articles     Next Articles

Effects of overexpression of CRELD1 gene on heart valve-related matrix proteins

CHEN Xuan1, GUO Ying2, YUAN Lang1, ZHANG Li1, HUANG Min1, SHEN Jie1   

  1. 1. Department of Cardiology, Shanghai Children's Hospital, Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China；2.Department of Cardiology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2012-03-28 Published:2012-03-28
  • Supported by:

    National Natural Science Foundation of China, 30772348；Science and Technology Foundation of Pudong New District

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Abstract:

Objective To investigate the effects of overexpression of CRELD1 gene on heart valve-related matrix proteins. Methods CRELD1 gene was obtained by PCR. Target gene eukaryote expression vectors were constructed by pcDNA3.1 (-) vector plasmid, and were identified by restriction enzyme digestion and DNA sequence analysis. Recombinant plasmid was transfected into human embryonic lung fibroblasts （HFL-I）with lipofectamine 2000 (recombinant plasmid group), and HFL-I cells transfected with empty vectors and those without transfection were served as transfection control group and blank control group respectively. Real-Time PCR and Western blotting were employed to detect the relative expression of mRNA and protein of heart valve-related matrix proteins Tenascin-C and Aggrecan in each group. Results DNA sequence of recombinant plasmid agreed very well with that in GenBank according to DNA sequence analysis. The relative expression of Aggrecan mRNA and protein in recombinant plasmid group was significantly lower than that in transfection control group and blank control group (P＜0.05), while there was no significant difference in the expression of Tenascin-C mRNA and protein among groups (P>0.05). Conclusion CRELD1 gene overexpression can decrease the heart valve-related matrix protein Aggrecan in human embryonic lung fibroblasts, which serves as a theoretical framework to demonstrate the roles of CRELD1 gene on atrioventricular septal defect.

Key words: CRELD1 gene, overexpression, Tenascin-C, Aggrecan, atrioventricular septal defect