›› 2012, Vol. 32 ›› Issue (3): 252-.doi: 10.3969/j.issn.1674-8115.2012.03.004

• Original article (Basic research) • Previous Articles     Next Articles

Effect of endoplasmic reticulum stress on expression of ubiquitin-fold modifier 1 in macrophages

LIU Hui-fang, ZHANG Hui-jie, LIU Xiao-yan, HU Qi-xian, MA Xiao-wen, HU Xiao-lei, CHEN Feng-ling   

  1. Department of Endocrinology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China
  • Online:2012-03-28 Published:2012-03-28
  • Supported by:

    National Natural Science Foundation of China, 30870954；Shanghai Jiaotong University School of Medicine Foundation, YZ1054, 09XBR01；Foundation of the Third People's Hospital, Shanghai Jiaotong University School of Medicine, syz2010-02

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Abstract:

Objective To investigate the effect of endoplasmic reticulum stress (ERS) on expression of ubiquitin-fold modifier 1 (Ufm1) in macrophages. Methods Peritoneal macrophages were obtained from mice with diabetes (db/db mice, diabetes group, n=6) and wild type (WT) C57 mice （control group, n=6）, and the expression of ERS-related factors (GRP78 and XBP1) and Ufm1 mRNA was detected by Real-Time PCR. RAW264.7 cells were induced with 8 μg/mL tunicamycin (TM), 0.5 μmol thapsigargin (TG) and 0.8 μmol TG separately, the expression of GRP78, XBP1 and Ufm1 mRNA was determined by Real-Time PCR, and the expression of ERS-related factors (p-eIf2α, eIf2α and CHOP) and Ufm1 protein was detected by Western blotting. Results The expression of GRP78, XBP1 and Ufm1 mRNA in peritoneal macrophages of diabetes group was significantly higher than that of control group (P<0.001). After induction by TM or TG, the expression of Ufm1 mRNA and protein in RAW264.7 cells was significantly higher than that in negative controls (P<0.05). Conclusion The expression of both ERS-related factors and Ufm1 in peritoneal macrophages increases in mice with diabetes. ERS in macrophages induced in vitro may increase the expression of Ufm1, which indicates that Ufm1 may participate in diabetic atherosclerosis by influencing function of macrophages partially through ERS pathway.

Key words: endoplasmic reticulum stress, macrophage, ubiquitin-fold modifier 1