›› 2013, Vol. 33 ›› Issue (2): 145-.doi: 10.3969/j.issn.1674-8115.2013.02.004

• Original article (Basic research) • Previous Articles     Next Articles

Anti-skin aging effect of GX-50

HOU Xue-feng1, SONG Xiao-fei2, WANG Zhao-xia1, DAI Jing-bo1, TANG Mao-ping1, CHEN Xiao-hui1, SONG Xiao-jing3, GAO Ping1, QIAO Zhong-dong1   

  1. 1.School of Life Sciences and Technology, Shanghai Jiaotong University, Shanghai 200240, China; 2.Department of Urology, Minhang Central Hospital of Ruijin Group, Shanghai 201100, China;3.Department of Cytology, Shanxi Medical University, Taiyuan 030001, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    530 Project of Wuxi, Jiangsu Province, 2011JDZX020


Objective To investigate the protective effect of GX-50 against skin aging. Methods GX-50 was applied on the back of mice aged 20 months, and the effect of GX-50 on mouse skin was observed with HE staining and immunohistochemistry. Human skin fibroblasts were primarily cultured, senescent cell model was established by H2O2 induction, and blank control group, DMSO group, GX-50 group, H2O2 group, and GX-50+H2O2 group (pretreatment with GX-50+H2O2 treatment) were divided. The anti-aging effect of GX-50 on primary human skin fibroblasts was examined by SA-βGal staining, as well as determination of secreted malondialdehyde (MDA) concentration and intracellular reactive oxygen species (ROS) content. The irritation characteristics of GX-50 were detected by Draize eye test. Results The histological observation revealed that the GX-50 treated skin exhibited better appearance and higher collagen content. β-galactosidase in human skin fibroblasts was reduced by GX-50. Compared with blank control group, the secretion of MDA was down-regulated by 30.45% and the fluorescence value of ROS was down-regulated by 8.03% in GX-50 group (P<0.05). Compared with H2O2 group, the secretion of MDA was down-regulated by 61.46% and the fluorescence value of ROS was down-regulated by 8.36% in GX-50+H2O2 group (P<0.05). Draize eye test showed the irritation score of GX-50 was 0. Conclusion GX-50 has protective and anti-aging effect on skin, and may serve as a potential cosmetic additive against skin aging.

Key words: GX-50, skin aging, collagen, malondialdehyde, reactive oxygen species