Abstract:

Objective To generate testis expressed gene 101 (Tex101) conditional knockout mice model, and to identify the phenotype. Methods The conditional knockout vector targeting exon 2 and exon 3 of Tex101 were generated by molecular cloning, and then the electroporation of embryonic stem (ES) cells was carried out. The targeted ES cells were screened by positive-negative selection and PCR, and the correct targeted ES cells were microinjected into blastula of C57BL/6J mice. Chimerical mice were generated after transplanted the blastula into the host mice, and then the Tex101-LoxP transgenic mice were generated. Tex101 cKO mice could be obtained by crossing between EⅡa-Cre mice and Tex101-LoxP transgenic mice. The genotype was identified by PCR and phenotype by frozen section. Results We got 2 male Tex101 cKO mice and the frozen section showed no discrepancy of testis morphology between WT and cKO mice. Also, there was no obvious difference of the testis weight. Conclusion The Tex101 conditional knockout mice were successfully obtained, which showed no obvious influence on the testis. This animal model provided the foundation for further study of Tex101 in vivo.

Key words: Tex101, conditional knockout, embryonic stem cell, EⅡa-Cre transgenic mouse