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Expression and biologic effects of microRNA-375 in triple negative breast cancer

YANG Chen1, YU Pei2, LI Ji-yu3, LOU Gu-yin4, XI Wen-qi1   

  1. 1.Department of Medical Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3.Department of Breast and Thyroid Surgery, Provincial Hospital Affilliated to Shandong University, Jinan 250021, China; 4.Department of Chemistry and Radiation Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2014-11-28 Published:2014-12-02
  • Supported by:

    Cultivation Program for Young Teachers in Shanghai Colleges, 2012-046

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Abstract:

Objective To investigate the expression and biologic effects of microRNA-375 (miRNA-375) of triple negative breast cancer (TNBC). Methods The expression of miRNA-375 of tumor tissue and adjacent normal tissues of 62 patients with TNBC was detected by the Real-time PCR. The relationship of the expression of miRNA-375 and clinicopathological features was analyzed. The expressions of miRNA-375 in breast cancer cell line MDA-MB-231 and breast fibroadenoma cell line MCF-10A were detected. The miRNA-375 precursor molecule was transfected to MDA-MB-231. The variations of the proliferation and invasion of MDA-MB-231 were detected by CCK-8 kit and Transwell test. Results The expression of miRNA-375 of TNBC tissue was significantly lower than that of adjacent normal tissue (P<0.001) and was relevant to the tumor size and axillary lymph node metastasis (P<0.001). Compared to MCF-10A, the expression of miRNA-375 in MDA-MB-231 significantly decreased (P=0.021). After being transfected by the miRNA-375 precursor, the expression of miRNA-375 in MDA-MB-231 significantly increased and the proliferation and invasion abilities decreased. Conclusion The expression of miRNA-375 in TNBC tissue and MDA-MB-231 is down-regulated. And miRNA-375 can inhibit the carcinogenesis and development of TNBC to a certain extent.

Key words: triple negative breast cancer, microRNA-375, invasiveness