• Original article (Basic research) • Previous Articles     Next Articles

Effects of targeted knockout of JARID2 gene on growth and migration of uveal melanoma cells

LIU Bo1,2, QIU Chun2*, LI Peng2, BI Jian-jun2, WU Qing2, NIU Bei-bei1   

  1. 1.Scientific Research Center, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine，Shanghai  200092, China; 2.Laboratory of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2015-05-28 Published:2015-06-04
  • Supported by:

    National Natural Science Foundation of China， 81372469， 81172323

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Abstract:

Objective To investigate the role of JARID2 gene in the growth and migration of uveal melanoma cells. Methods The mRNA expression of JARID2 in uveal melanoma cell lines OM431 and SP6.5 was detected by the real-time PCR. The JARID2 gene of OM431 and SP6.5 was knocked out by shRNA and stable transfected cell lines shSP6.5 and shOM431 were constructed. Variations of protein expression of JARID2 were detected by the Western blotting. Effects of targeted knockout of JARID2 gene on the growth, migration, and in vitro colony formation of uveal melanoma cells were observed. Results The mRNA expression of JARID2 in OM431 and SP6.5 was over-expressed. The results of Western blotting showed that compared with OM431 and SP6.5, the protein expression of JARID2 in shOM431 and shSP6.5 significantly decreased. Targeted knockout of JARID2 gene could inhibit the growth, migration, and in vitro colony formation of uveal melanoma cells. Conclusion JARID2 gene plays an important role in the growth, migration, and colony formation of uveal melanoma cells.

Key words: JARID2 gene, uveal melanoma, tumor metastasis