›› 2017, Vol. 37 ›› Issue (8): 1079-.doi: 10.3969/j.issn.1674-8115.2017.08.006

Previous Articles     Next Articles

Gender dependence of metabolomic phenotypes for human saliva using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry

LI Jing1, 2, LIU Si1, 2, LEI He-hua1, WANG Yu-lan1#, TANG Hui-ru3#   

  1. 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. School of Life Sciences, Fudan University, Shanghai 200438, China
  • Online:2017-08-28 Published:2017-09-28

Abstract: Objective · To investigate the gender-related metabolomic differences in human saliva.  Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences.  Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites, 7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine, isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males.  Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva.

Key words: saliva, metabolomics, ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry, gender dependence