›› 2019, Vol. 39 ›› Issue (5): 458-.doi: 10.3969/j.issn.1674-8115.2019.05.004

• Original article (Basic research) • Previous Articles     Next Articles

Effects of bisphenol A exposure during embryonic phase on development and neurobehavior of zebrafish

PAN Rui1, HU Jing-ying2, HU Yi3, YAO Qian1, LI Wei-hua2, TIAN Ying1, 4, GAO Yu1   

  1. 1. Shanghai Jiao Tong University School of Public Health, Shanghai 200025, China; 2. National Health Commission Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Reproduction and Developmental Research Institute of Fudan University, Shanghai 200032, China; 3. Center for Medical Bioinformatics, Shanghai Childrens Hospital, Shanghai Jiao Tong University, Shanghai 200040, China; 4. MOE and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2019-05-28 Published:2019-07-26
  • Supported by:
    National Natural Science Foundation of China, 81872629; Natural Science Foundation of Shanghai, 17ZR1415800; Open Subject of Ministry of Education and Shanghai Key Laboratory of Environment and Children Health, CEH2018072

Abstract: Objective · To investigate the effects of bisphenol A (BPA) on the development and neurobehavior of zebrafish. Methods · The zebrafish fertilized eggs that developed to 4 hpf (hours post-fertilization) were treated with different concentrations of BPA (0, 2.5, 25, 250, and 2 500 μg/L). Survival rates, malformation rates, hatching rates, 24 hpf autonomic movement, and 48 hpf heart rates in the groups were observed and recorded. And the neurobehavioral test of larval zebrafish that developed to 144 hpf was performed. Results · The BPA exposure did not affect the survival rates, malformation rates and hatching rates of zebrafish. Compared with the control group, the autonomic movement in 24 hpf and heart rate in 48 hpf of zebrafish embryos that exposed to 2 500 μg/L BPA decreased (P0.000), and the total distance and average speed of larval zebrafish that developed to 144 hpf also decreased (P0.000). The behavior of larval zebrafish exposed to 250 μg/L and 2 500 μg/L BPA changed on light-evoked startle escape response activity, mainly including the decrease of total distance under strong light stimulation (P<0.05). Conclusion · BPA exposure during embryonic phase has developmental toxicity and neurotoxicity on zebrafish.

Key words: bisphenol A, zebrafish, embryonic phase, development, neurobehavior

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