›› 2019, Vol. 39 ›› Issue (6): 571-.doi: 10.3969/j.issn.1674-8115.2019.06.003

• Original article (Basic research) • Previous Articles     Next Articles

Effects of lutein on transforming growth factor-β2 induced epithelial-mesenchymal transition in ARPE-19 cells

Lü Ya-nan, GU Qing, LI Dong-li, GONG Yuan-yuan   

  1. Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Key Laboratory of Ocular Fundus Disease, Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
  • Online:2019-06-28 Published:2019-07-26
  • Supported by:
    National Key R&D Plan of China, 2016YFC0904800; Clinical Research Innovation Plan of Shanghai General Hospital, CTCCR-2018BP04）。

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Abstract: Objective · To establish the transforming growth factor-β2 (TGF-β2) induced epithelial-mesenchymal transition (EMT) model of retinal pigment epithelium cells, and investigate the effect and mechanism of lutein on EMT. Methods · ARPE-19 cells were cultured and divided into 4 groups including control group, TGF-β2 group, TGF-β2+lutein group and lutein group. The mRNA levels of α-smooth muscle actin (α-SMA), fibronectin (FN) and E-cadherin were analyzedreal-time PCR. The protein of α-SMA, FN and occludin were assayedWestern blotting. Immunofluorescence was used to detect the change of α-SMA. Meanwhile, Western blotting was performed to detect the levels of pSmad3 in the TGF/Smad signaling pathway. Results · TGF-β2 induced EMT was inhibitedlutein. Lutein decreased the mRNA and protein levels of the mesenchymal markers α-SMA and FN, and increased the of the epithelial markers E-cadherin and occludin (all P<0.05). Immunofluorescence showed that lutein can inhibit the conversion of epithelial cells into myofibroblasts. Lutein significantly downregulated the high of pSmad3 in TGF-β2 treated ARPE-19 cells (P0.001). Conclusion · Lutein inhibits TGF-β2 induced EMTdownregulating the of pSmad3 in TGF-β/ Smad signaling pathway, indicating it may attenuate subretinal fibrosis.

Key words: subretinal fibrosis, lutein, transforming growth factor-&, beta, 2 (TGF-&, beta, 2), epithelial-mesenchymal transformation (EMT), retinal pigment epithelium cell (RPE)

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