Role and clinical significance of intercellular adhesion molecule-1 in CD4+ T cells assisting B cells to produce antibodies

doi:10.3969/j.issn.1674-8115.2021.05.005

Abstract

Abstract: Objective

·To investigate the role of intercellular adhesion molecule-1 (ICAM-1) in CD4⁺ T cells assisting B cells to produce antibodies, and the correlation between ICAM-1 and systemic lupus erythematosus (SLE).

Methods

·①From October 2018 to May 2020, 50 SLE patients (SLE group) of Rheumatology Department and 60 healthy people (HC group) of medical examination center from Renji Hospital, Shanghai Jiao Tong University School of Medicine were recruited. Flow cytometry was used to analyze the expression of ICAM-1 on the surface of CD4⁺ T cells in peripheral blood of the two groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the soluble ICAM-1 (sICAM-1) level in serum of the two groups. ②Peripheral blood mononuclear cells (PBMCs) of healthy people were isolated by density gradient centrifugation, and activated by α-CD3/28 in vitro at 24, 48 and 72 h, respectively. The expression of ICAM-1 on the surface of CD4⁺ T cells was analyzed by flow cytometry, and the level of sICAM-1 in the supernatant was detected by ELISA. ③CD4⁺ T cells and B cells of healthy people were co-cultured in vitro, which were divided into control group, stimulation group and antibody blockade group. After 12 days, the IgG level in the co-culture supernatant of the three groups was detected by ELISA.

Results

·①The expression of ICAM-1 on the surface of CD4⁺ T cells in peripheral blood and the level of sICAM-1 in serum of the SLE group was higher than that of the HC group (both P<0.05). Correlation analysis showed that ICAM-1 expression on the surface of CD4⁺ T cells was positively correlated with erythrocyte sedimentation rate, and serum sICAM-1 level was positively correlated with anti-dsDNA antibody and IgG level (all P<0.05). ②The expression of ICAM-1 on the surface of CD4⁺ T cells and the level of sICAM-1 in the supernatant of healthy people were significantly up-regulated gradually along with the stimulation α-CD3/28. ③After co-culture, the IgG level in the supernatant of the stimulation group was higher than that in the control group, while IgG lever in antibody blockade group was lower than that in the stimulation group (both P<0.05).

Conclusion

·ICAM-1 can promote the production of IgG after the interaction of CD4⁺ T cells and B cells, which can be used as a potential target for the treatment of SLE.

Key words: systemic lupus erythematosus (SLE), CD4⁺ T cell, B cell, antibody production, intercellular adhesion molecule-1 (ICAM-1)

CLC Number:

R392.12

Qian ZHAO, Mei-yu ZHANG, Ping JI, Jia-yuan WANG, Shu-jun WANG, Shuai LIU, Ying WANG. Role and clinical significance of intercellular adhesion molecule-1 in CD4⁺ T cells assisting B cells to produce antibodies[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(5): 588-594.

Figures/Tables 7

Tab 1 Basic data and laboratory test indexes of the two groups

Item	HC group (n=60)	SLE group (n=50)
Basic data
Age/year	42.3±2.1	39.5±1.9
Gender(male/female)/n	25/35	4/46
Hemoglobin/(g·L^-1)	153.32±11.01	119.10±22.01
Laboratory test indexes
Course of disease/d	?	41.5 (21.0, 77.5)
ESR/(mm·h^-1)	?	27.35±23.00
anti-dsDNA antibody/(U·mL^-1)	?	54.60±36.55
ANA	?	1∶640
C3 level/(g·L^-1)	?	0.71±0.28
C4 level/(g·L^-1)	?	0.11±0.05
IgG level/(g·L^-1)	?	9.65±2.34
IgM level/(g·L^-1)	?	0.58±0.13
SLEDAI/score	?	6.33±5.42

Fig 1 Expression of ICAM-1 on peripheral blood CD4+ T cells in the SLE group and HC group

Fig 2 Correlation between ICAM-1 expression on the surface of CD4+ T cells and peripheral pathological factors in SLE patients

Fig 3 Analysis of serum sICAM-1 levels in the SLE group and HC group

Fig 4 Correlation between serum sICAM-1 level and peripheral pathological factors in SLE patients

Fig 5 Expression of ICAM-1 on the surface of CD4+ T cells and the content of sICAM-1 in culture supernatant with different stimulation time

Fig 6 Expression of IgG in the supernatant of CD4+ T cells-B cells co-culture in vitro

TrendMD

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