Abstract:

Objective To establish murine bone marrow mesenchymal stem cell lines that can stably express the luciferase. Methods OP9 cells of MSC lines were infected by lentivirus GV260 containing luciferase gene and screened by puromycin for establishing OP9 luc+ cell lines that could stably express the luciferase. The activity of reporter gene of luciferase was detected and its expression effect was observed. OP9 luc+ cells were injected via the tail vein of C57BL mice and imaging ability was observed. Results OP9 luc+ cells that stably expressed luciferase were screened. The activity detection proved that the expression efficiency of OP9 luc+ cells was high. OP9 luc+ cells could form image clearly in vivo. Conclusion Murine bone marrow mesenchymal stem cell lines with stable and high expression of luciferase are established.

Key words: luciferase, optical in vivo imaging, bioluminescence, bone marrow mesenchymal stem cell