Inhibitory effects of different isoforms of DLC1 on proliferation and migration of colon cancer cells

doi:10.3969/j.issn.1674-8115.2016.01.004

• Original article (Basic research) • Previous Articles Next Articles

Inhibitory effects of different isoforms of DLC1 on proliferation and migration of colon cancer cells

PENG Hai-xia1,2, HOU Ming-yue3, CHU Yi-min2, YANG Da-ming2, ZHOU Feng-li2, KUAI Rong2, #br# XU Ying2, HOU Zhao-yuan4, LI ji2, JIN Zhen-dong1

1.Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2.Digestive Endoscopy Center, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; 4. Peoples Hospital of Lanling, Lanling 277799, China; 5.Department of Biochemistry & Molecular Cellular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online:2016-01-28 Published:2016-02-26
Supported by:
National Natural Science Foundation of China, 81372028; Project of Shanghai Municipal Commission of Health and Family, 201440426; Scientific Research Project of Shanghai Sixth People's Hospital, ly201301。

Abstract

Abstract:

Objective To investigate the inhibitory effect of different isoforms of deleted in liver cancer-1 (DLC1) on the proliferation and migration of the colon cancer cell line. Methods The expressions of isoforms of DLC1 in 17 colon cancer tissue samples and 17 normal tissue samples were detected by qRT-PCR. Colon cancer cells SW1116 were infected by over-expressed lentiviruses of different isoforms. The effects of different isoforms on the proliferation and migration of the colon cancer cell line were observed by CCK8 kit and Transwell test. Results The expressions of different isoforms of DLC1 in colon cancer tissues were significantly lower than those of adjacent normal tissues. Over-expression of DLC1 could inhibit the proliferation and migration of the colon cancer cell line SW1116 and the inhibitory effect of isoform2 (DLC1-isoform2) was the strongest. Conclusion The expressions of different isoforms of DLC1 in colon cancer tissues are significantly lower than those of adjacent normal tissues. The inhibitory effect of DLC1-isoform2 of DLC1 on the proliferation and migration of colon cancer cells is the strongest.

Key words: colon cancer, deleted in liver cancer-1, isoform, proliferation, migration

PENG Hai-xia, HOU Ming-yue CHU Yi-min, et al. Inhibitory effects of different isoforms of DLC1 on proliferation and migration of colon cancer cells[J]. , doi: 10.3969/j.issn.1674-8115.2016.01.004.

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Inhibitory effects of different isoforms of DLC1 on proliferation and migration of colon cancer cells

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