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Effects of recombinant human amelogenin on expressions of inflammatory factors of human periodontal ligament fibroblasts under inflammatory microenvironment

LIAO Wei-wen, SONG Zhong-chen, SHU Rong, DONG Jia-chen   

  1. Department of Periodontology, College of Stomatology, Shanghai Key Lab of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    National Natural Science Foundation of China, 81271156。

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Abstract:

Objective To investigate the effects of recombinant human amelogenin (rhAm) on expressions of inflammatory factors of human periodontal ligament fibroblasts (hPDLCs) cultured in vitro under inflammatory microenvironment. Methods hPDLCs were cultured in vitro and identified by immunohistochemical staining method. The inflammatory microenvironment was simulated by lipopolysaccharide (LPS) with Porphyromonas gingivalis  of 10 μg/mL and hPDLCs were treated by rhAm of 20 μg/mL. Expressions of inflammatory factors interleukin-1β (IL-1β), IL-6, and IL-8 were detected by real-time PCR and ELISA. Results Results of immunohistochemical staining confirmed that cultured cells were hPDLCs. Expressions of IL-1β, IL-6, and IL-8 could be induced by LPS with P. gingivalis of 10 μg/mL at four time points (6, 12, 24, 72 h). Expressions of inflammatory factors decreased after being treated by rhAm of 20 μg/mL. Conclusion The rhAm can inhibit expressions of inflammatory factors of hPDLCs under inflammatory microenvironment.

Key words: periodontal ligament cell, inflammatory microenvironment, recombinant human amelogenin, inflammatory factor