Abstract:

Objective To investigate the protective effect of resveratrol (RSV) on lipopolysaccharide (LPS)-induced damage of H9c2 cells and relevant mechanisms. Methods Mouse myocardial cells H9c2 were treated by LPS of 10 μg/mL for 0, 5, 10, 20, 30, and 60 min. The inductive effect of LPS on the expressions of nuclear factor κB inhibitory protein (IκBα), p-p65, p65, and β-actin at different time points was evaluated. H9c2 cells were stimulated by LPS after being pre-treated by different concentrations of RSV and the effects of RSV on the LPS-induced damage of H9c2 cells were observed. The proliferation of H9c2 cells was detected by MTT. The intracellular oxygen species reactive (ROS) level was detected by fluorescence method. The expressions of IκBα, p-p65, p65, and β-actin were detected by Western blotting. Results LPS inhibited the proliferation of H9c2 cells and the intracellular ROS level significantly elevated. LPS significantly decreased the expression level of IκBα and significantly increased the phosphorylation of p65. Pretreatment by RSV remarkably reduced the LPS-induced damage of H9c2 cells and significantly decreased the intracellular ROS level. RSV up-regulated the expression of IκBα and down-regulated the expression of p-p65. Conclusion RSV may inhibit the LPS-induced damage of H9c2 cells via down-regulating the expression of p-p65 and up-regulating the expression of IκBα.

Key words: resveratrol, lipopolysaccharide, p65, IκBα, oxygen species reactive, H9c2 cardiomyocytes