›› 2018, Vol. 38 ›› Issue (2): 138-.doi: 10.3969/j.issn.1674-8115.2018.02.004

• Original article (Basic research) • Previous Articles     Next Articles

Influence of 3,3’-diindolylmethane on expression of inflammatory cytokines in periodontal ligament cells induced by lipopolysaccharide

ZHOU Ju-mei1, YUAN Ke-yong2, LIN Wen-zhen2, HU Xu-chen2, JIN Qiao-qiao2, NIU Chen-guang2   

  1. 1. Department of Stomatology, Kunshan Third People’s Hospital, Kunshan 215316, China; 2. Department of Endodontics, Shanghai Ninth People’s  Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai 200011, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81570964；Project of Shanghai Municipal Committee of Science and Technology, 1714090350

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Abstract: Objective · To investigate the effect of 3,3’-diindolylmethane (DIM) on the expression of inflammatory cytokines in human periodontal ligament cells (hPDLCs) induced by lipopolysaccharide (LPS) and to study the related mechanism. Methods · hPDLCs were isolated and cultured, and
CCK-8 method was used to detect the effect of DIM on the proliferation of hPDLCs. hPDLCs were randomly divided into 4 groups: blank group (without
LPS and DIM), LPS group (10 μg/mL LPS), 10 μg/mL LPS+6.25 μg/mL DIM, 10 μg/mL LPS+12.50 μg/mL DIM. The cells of all groups were cultured
for 12 h. The protein levels of TNF-α, IL-1β and IL-6 in supernatant were detected by enzyme linked immunosorbent assay. The change of mitogenactivated protein kinase (MAPK) and nuclear factor κB (NF-κB) signaling pathways were detected by Western blotting. Results · The cell viability was
not affected when the DIM concentration was less than 50 μmol/L (P>0.05). DIM at 6.25 and 12.50 μg/mL reduced the LPS-induced expression of TNF-α,
IL-1β and IL-6 at protein levels (P<0.05). DIM inhibited the activation of the NF-κB signaling pathway. Conclusion · DIM can reduce the LPS-induced
inflammatory cytokine expression in hPDLCs via restraining the activation of the NF-κB signaling pathway.

Key words: human periodontal ligament cells, 3,3’-diindolylmethane, inflammatory cytokine, nuclear factor κB