›› 2018, Vol. 38 ›› Issue (7): 769-.doi: 10.3969/j.issn.1674-8115.2018.07.009

• Original article (Clinical research) • Previous Articles     Next Articles

Relationship between global DNA methylation and hydroxymethylation levels in peripheral blood of elderly patients with myocardial infarction and the degree of coronary atherosclerosis

HU Chun-xiao, JIANG Dan, WU Shi-yong, GAO Lei, CHANG Guang-lei, TAO Hong-mei, ZHANG Dong-ying   

  1. 1. Department of Cardiovascular Medicine, 2. Department of Laboratory Research Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2018-07-28 Published:2018-07-30
  • Supported by:
    National Natural Science Foundation of China, 81570212; Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission, ZY201702073; Postgraduate Research and Innovation Project of Chongqing, CYB15093; Science and Technology Plan Project of Yuzhong District of Chongqing, 20140111; Cardiac Rehabilitation and Metabolic Therapy Research Foundation; “Advanced” Research Foundation Project of Cardiovascular Health Institute, China Cardiovascular Health Alliance

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Abstract: Objective · To investigate the relationship between the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with myocardial infarction (MI) and the degree of coronary atherosclerosis. Methods · Forty-three elderly patients with MI were enrolled as the study group and 53 elderly patients with negative findings in coronary angiograms during the same period were collected as the control group. Peripheral blood samples were collected. The levels of global DNA methylation and hydroxymethylation in peripheral blood and the concentration of plasma TET2 were detectedELISA. TET2 mRNA was determinedreal-time qPCR. Results · Compared with the control group, the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI were significantly increased (P0.014, P0.000). The concentration of plasma TET2 and its mRNA in patients with MI were significantly higher than those in the control group (P0.004, P0.028).The levels of global DNA methylation and hydroxymethylation in MI group were significantly correlated with the degree of coronary atherosclerosis (r0.262, P0.017; r0.360, P0.001). Conclusion · The levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI are significantly increased, which are significantly correlated with the degree of coronary atherosclerosis.

Key words: elderly, myocardial infarction, methylation, hydroxymethylation

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