›› 2018, Vol. 38 ›› Issue (11): 1283-.doi: 10.3969/j.issn.1674-8115.2018.11.002

• Original article (Basic research) • Previous Articles     Next Articles

Characterization and genome sequencing of SH-Ab 15599, a novel bacteriophage targeting carbapenem-resistant Acinetobacter baumannii

JI Jia-wei1, 2, WANG Rui1, LUO Ting-ting4, XU Meng-sha1, GUO Xiao-kui1, HU Fu-pin3, LI Min2, HE Ping1   

  1. 1. Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China; 2. Department of Laboratory Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China; 4. Department of Pharmaceutical Engineering, Zhejiang Pharmaceutical College, Ningbo 315100, China
  • Online:2018-11-28 Published:2018-12-15
  • Supported by:
    National Natural Science Foundation of China, 81471908

Abstract: Objective · To find lytic bacteriophages which have potential to combat carbapenem-resistant Acinetobacter baumannii (CRAB) infections and analyze the biological characterizations and genomic sequences of the phages. Methods · The phages were isolated sewage followedamplification and purification. They were characterizedthe transmission electron microscopy, the host specificity, the biological characterizations, the lysis assay in vitro and the genome sequence analysis. Results · A novel bacteriophage, SH-Ab 15599, was isolated sewage. The electron microscope revealed SH-Ab 15599 belongs to Myoviridae. Among the 51 clinical CRAB isolates tested, 15 (29.4%) were lysedthe phage. SH-Ab 15599 was relatively sat temperature range 4 ℃ to 50 ℃ or between pH 6 and 9. About 90% of the phage was approximately adsorbed to the host CRAB isolate in 5 min. The one-step growth curve showed that the latent period was about 10 min and the growth period was about 20 min. The burst size was estimated at 43 pfu per infected cell. The growth of the host CRAB isolate was inhibitedthe phage in vitro. The genome of SH-Ab 15599 was a linear, 143 204 bp double-stranded DNA with GC content of 38.45%. Conclusion · SH-Ab 15599 is a novel phage targeting CRAB and has potential as an alternative against CRAB infections in the future.

Key words: Acinetobacter baumannii, carbapenem-resistant Acinetobacter baumannii, bacteriophage, biological characteristics, genomics

CLC Number: