›› 2019, Vol. 39 ›› Issue (3): 258-.doi: 10.3969/j.issn.1674-8115.2019.03.007

• Original article (Basic research) • Previous Articles     Next Articles

Effect of transforming growth factor β signaling on the stem cell-mediated liver regeneration in mice after partial hepatectomy

CHEN Zi-xin1, JIN Xin1, WAN Li1, LI De-wei2   

  1. 1. Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2019-03-28 Published:2019-04-28
  • Supported by:
    National Natural Science Foundation of China, 81470898

Abstract: Objective · To explore the effect of inhibiting transforming growth factor β (TGF-β) signaling pathway on the stem cell-mediated liver regeneration in mice after partial hepatectomy (PH). Methods · Eighteen C57BL/6 male mice were selected to establish the model of hepatectomy. mRNA and protein levels of the signal molecules in TGF-β pathway, as well as stem cell markers α-fetoprotein (AFP) and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) in the liver tissues were detected before (PH0) and on the 1st (PH1), 3rd (PH3) and 7th (PH7) day after operation. Then, additional 32 mice were assigned into inhibitor group [PH+SB-431542, 10 mg/(kg·d)] and control group (PH+normal saline). The mice were sacrificed on the 1st (PH1) and 3rd (PH3) day after operation and liver remnants were obtained. Realtime-PCR, Western blotting, and immunofluorescence staining were used to detect the expressing variation of TGF-β signaling pathway, AFP and LGR5. Results · The mRNA and protein of TGF-β1, phosphorylated SMAD2 (p-SMAD2) protein, Afp mRNA, the number of AFP immune-positive cells and Lgr5 mRNA were upregulated significantly after PH, peaked at PH3 (P<0.05) and recovered to pre-operative level at PH7. Compared with control group, the above indices were all obviously inhibited in inhibitor group at PH3 (P<0.05). Conclusion · TGF-β signaling pathway may regulate the stem cell-mediated liver regeneration in mice after PH.

Key words: partial hepatectomy (PH), transforming growth factor &, beta, (TGF-&, beta, ), stem cell, liver regeneration, &, alpha, -fetoprotein (AFP)

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