›› 2009, Vol. 29 ›› Issue (7): 817-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of deep brain stimulation on expression of DARPP-32 and its phosphorylated proteins in corpus striatum of rats with dyskinesia

PU Zheng, LU Li-xia, LIU Zhen-guo   

  1. Department of Neurology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2009-07-25 Published:2009-09-16
  • Supported by:

    Scientific Research Foundation of the Ministry of Education for Returned Overseas Chinese Scholars; Shanghai Education Committee Foundation, 06BZ048; Shanghai Pujiang Talent Program; Shanghai Science and Technology Committee Foundation, 07SP07005

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Abstract:

Objective To investigate the effects of subthalamic nucleus (STN)-deep brain stimulation (DBS) on expression of dopamine and adenosine 3’5’-nophosphate-egulated phosphor-rotein (DARPP-32) and its phosphorylated proteins in corpus striatum of rat models with levodopa-nduced dyskinesia. Methods The rat models of levodopa-nduced dyskinesia were set up and were given STN-DBS (stimulation group). The expression of DARPP-32 and its phosphorylated proteins in corpus striatum (damage side and normal side) were detected and compared with sham-stimulation group and sham-operation group. Results There was no significant difference in the expression of DARPP-32 total protein in corpus striatum of rats with dyskinesia among three groups (P>0.05). The expression of Phosphor-Thr34-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly lower than that in sham-stimulation group and sham-operation group（P<0.05）, while the expression of Phosphor-Thr75-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly higher than that in sham-stimulation group and sham-operation group（P<0.05）. Conclusion DARPP-32 and its phosphorylated proteins play an important role in the pathogenesis of levodopa-induced dyskinesia.

Key words: Parkinson´s disease, dyskinesia, deep brain stimulation, subthalamic nucleus, dopamine and adenosine 3’5’-monophosphate-regulated phosphor-protein