›› 2009, Vol. 29 ›› Issue (7): 813-.

• Original article (Basic research) • Previous Articles     Next Articles

Research on mechanism of chloroquine phosphate-induced U937 cell apoptosis

LIU Jia1, CHEN Fang-yuan1, WANG Hai-rong1, ZHONG Ji-hua1, WANG Li-ming2, ZHONG Hua1, HAN Jie-ying1, OUYANG Ren-rong1   

  1. 1. Department of Hematology, Shanghai Institute of Hematology, 2. Center Laboratory, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China
  • Online:2009-07-25 Published:2009-09-16
  • Supported by:

    National Natural Science Foundation of China, 30670881;Research Foundation of Renji Hospital-Shanghai Jiaotong University School of Medicine, ZD0704

Abstract:

Objective To observe the effects of chloroquine phosphate on apoptosis of leukemic cell line U937, and investigate whether chloroquine phosphate induces leukemic cell apoptosis by normalizing protein PNAS-2's abnormal subcellular location. Methods Chloroquine phosphate of different concentrations were added into culture fluid of leukemic cell line U937 at logarithmic phase. MTT was used to measure cell proliferation, flow cytometry and laser confocal microscopy were applied to detect cell apoptosis, and immunofluorescence technology was employed to observe the effects of chloroquine phosphate on the changes of subcellular location of protein PNAS-2. Results Apoptosis of leukemic cell line U937 was significantly induced by 50 μg/mL chloroquine phosphate, and subcellular location of protein PNAS-2 was changed. Conclusion Chloroquine phosphate can induce apoptosis of leukemic cell line U937, and the mechanism may be related to the normalization of PNAS-2's abnormal subcellular location in U937 cell line. Chloroquine phosphate has the potential to be used in leukemic therapy.

Key words: chloroquine phosphate, apoptosis, PNAS-2, leukemia, subcellular localization