›› 2010, Vol. 30 ›› Issue (2): 121-.

• Monographic report (Pediatric research) • Previous Articles     Next Articles

Effects of Epstein-Barr virus on apoptosis of cord blood monocytes-derived dendritic cells

JIN Ying-ying1, WANG Xi2, CHEN Tong-xin1,2   

  1. 1. Department of Pediatrics, 2. Department of Immunology/Oncology, Shanghai Institute for Pediatric Research, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007Y76；Foundation of Shanghai Jiaotong University School of Medicine, BXJ0919；Shanghai Science and Technology Committee Foundation, 044119628；Shanghai Education Committee Foundation, 082261；National Natural Science Foundation of China, 30471620

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Abstract:

Objective To investigate the effects of Epstein-Barr virus (EBV) infection on apoptosis of cord blood monocytes-derived dendritic cells (DC) in neonates. Methods Cord blood monocytes were induced into DC by 50 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) and 10 ng/mL recombinant human interleukin-4 (rhIL-4), and DC were divided into three groups: ①4G group: rhGM-CSF and rhIL-4 were added separately on the day of cell separation; ②4G+0d EBV group: rhGM-CSF, rhIL-4 and B95.8 cell supernatants were added simultaneously on the day of cell separation; ③4G+5d EBV group: hGM-CSF and rhIL-4 were added on the day of cell separation, and B95.8 cell supernatants were added on the 5th day. Percents of DC apoptosis were measured using Annexin V-FITC and PI staining by flow cytometry. The expression of X-linked inhibitor of apoptosis protein (XIAP) was detected by Western Blot. Results Percent of DC apoptosis in 4G+0d EBV group was significantly higher than that in 4G group on the 6th to 14th day (P<0.05), and percent of DC apoptosis in 4G+5d EBV group was significantly higher than that in 4G group on the 7th to 14th day (P<0.05). The effects of EBV infection on percent of DC apoptosis had relations with infection time points. The expression of XIAP in DC decreased significantly after EBV infection. Conclusion EBV promotes apoptosis of cord blood monocytes-derived DC, which is associated with the differentiation and maturation status of DC.

Key words: neonatal, cord blood, Epstein-Barr virus, dendritic cells, apoptosis