Abstract:

Objective To investigate the effects of dysfunction of anion exchanger 2 (AE2) on secretion of epithelial cells of colon mucosa and intestinal peristalsis. Methods SD rat constipation models were established by intragastric administration of diphenoxylate compound (model group, n=17), and SD rats without model establishment were served as controls (control group, n=28). Rat epithelial cells of colon mucosa were isolated and cultured in vitro, changes of pH after adding AE2 inhibitor DIDS into culture fluid of epithelial cells of colon mucosa were compared between model group and control group with single cell ion imaging. Curves of in vitro intestinal peristalsis were drawn with Powerlab System and Chart 5 Software, amplitudes and frequencies of in vitro intestinal peristalsis were compared between groups, and intestinal peristalsis function was evaluated. The changes of intestinal peristalsis function after adding AE2 inhibitor DIDS into culture fluid of in vitro intestine in control group were observed and compared with fast adding test. Results Single cell ion imaging revealed that pH in model group increased after addition of DIDS into culture fluid of epithelial cells of colon mucosa, and was significantly different from that in control group after addition of DIDS (P<0.01). It was obtained from curves of intestinal peristalsis that the amplitude and frequency of in vitro colon smooth muscle peristalsis in model group were significantly lower than those in control group (P<0.05), and fast adding test indicated that the amplitude and frequency of in vitro colon smooth muscle peristalsis in control group significantly decreased after addition of DIDS into culture fluid (P<0.05). Conclusion The dysfunction of AE2 may result in the disorder of secretion and peristalsis of colon.

Key words: anion exchanger 2, colon, peristalsis, secretion