›› 2011, Vol. 31 ›› Issue (1): 5-.doi: 10.3969/j.issn.1674-8115.2011.01.002

• Original article (Basic research) • Previous Articles     Next Articles

Expression of TLR3 in oral squamous carcinoma cells and induction of apoptosis by its ligand poly(I:C)

LUO Qing-qiong1, CHEN Wan-tao2, HU Shui-qing1, CHEN Fu-xiang1   

  1. 1.Department of Laboratory, 2.Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    Shanghai Pujiang Talent Program, 09PJ1407100;Foundation for Returned Overseas from The Ministry of Education, 20091001;Shanghai Natural Science Foundation, 08ZR1412700;Shanghai Key Discipline Foundation, S30206


Objective To investigate the expression of Toll-like receptor 3 (TLR3) and the biological characteristics of its ligand poly (I:C) in oral squamous carcinoma cells. Methods The expression of TLR3 mRNA and protein in oral squamous carcinoma CAL-27 cells and HB cells was detected by RT-PCR and flow cytometry. CAL-27 cells were treated with 100 μg/mL poly (I:C), cell viability was determined by MTT assay at 24, 48 and 72 h after stimulation, and cell apoptosis was detected by flow cytometry. Besides, blank controls were established. Results TLR3 mRNA and protein were highly expressed in CAL-27 and HB cells. After treatment by poly (I:C), the viability of CAL-27 cells was significantly lower than that of blank controls at each time point (P<0.05), and the apoptosis rate of CAL-27 cells was significantly higher than that of blank controls [(16.67±1.202)% vs (7.00±1.155)%](P<0.05). Conclusion TLR3 is highly expressed in oral squamous carcinoma cells, and its ligand poly(I:C) can induce the apoptosis of oral squamous carcinoma cells.

Key words: poly (I:C), Toll-like receptor, oral squamous carcinoma, apoptosis