›› 2011, Vol. 31 ›› Issue (2): 187-.doi: 10.3969/j.issn.1674-8115.2011.02.015

• Original article (Basic research) • Previous Articles     Next Articles

Mechanism of apoptosis induced by Resveratrol |in human gastric cancer HGC27 cells

YANG Hong-mei, XING Ying, ZHENG Mei-zhen   

  1. Department of Internal Medicine, Provincial Tumor Hospital of Liaoning, Shenyang 110042, China
  • Online:2011-02-28 Published:2011-03-01


Objective To investigate the relationship between the expression of VEGF and MMP-9 in PTEN and p-Akt signaling pathway of resveratrol affecting gastric cancer HGC27 cells. Methods The effects of resveratrol on growth inhibition rate and cell cycle of HGC27 cells were examined by CCK-8 test and flow cytometry, respectively. The effects of resveratrol on apoptosis-related PTEN and p-Akt gene in HGC27 cells were detected by Western blotting, and the contents of VEGF and MMP-9 protein in HGC27 cells before and after resveratrol treatment were determined by ELISA. Results The growth inhibition rate of HGC27 cells by resveratrol treatment was concentration- and time-dependent (P<0.05). Forty-eight hours after resveratrol treatment, cell cycle analysis indicated that the percent of HGC27 cells with DNA synthesis (S phase) significantly decreased, which exhibited a negative correlation with dosage (r=-0.930, P=0.007). There was a positive relationship between the relative expression of PTEN and Res concentration (r=0.951,P=0.04), while there was a negative relationship between the relative expression of p-Akt, VEGF and MMP-9 and Res concentration (r=-0.969,P=0.03;r=-0.894,P=0.02;r=-0.881,P=0.01). Conclusion Resveratrol could inhibit the proliferation and induce apoptosis of human gastric cancer HGC27 cells, which may be related to the expression up-regulation of PTEN and phosphorylation inhibition of Akt. Resveratrol could also regulate the expression of VEGF and MMP-9 to inhibit the proliferation and apoptosis of HGC27 cells.

Key words: resveratrol, HGC27 cell, apoptosis, PTEN, p-Akt