Abstract:

Objective To investigate the relationship between the expression of VEGF and MMP-9 in PTEN and p-Akt signaling pathway of resveratrol affecting gastric cancer HGC27 cells. Methods The effects of resveratrol on growth inhibition rate and cell cycle of HGC27 cells were examined by CCK-8 test and flow cytometry, respectively. The effects of resveratrol on apoptosis-related PTEN and p-Akt gene in HGC27 cells were detected by Western blotting, and the contents of VEGF and MMP-9 protein in HGC27 cells before and after resveratrol treatment were determined by ELISA. Results The growth inhibition rate of HGC27 cells by resveratrol treatment was concentration- and time-dependent （P<0.05）. Forty-eight hours after resveratrol treatment, cell cycle analysis indicated that the percent of HGC27 cells with DNA synthesis (S phase) significantly decreased, which exhibited a negative correlation with dosage （r=－0.930, P=0.007）. There was a positive relationship between the relative expression of PTEN and Res concentration （r=0.951，P=0.04）, while there was a negative relationship between the relative expression of p-Akt, VEGF and MMP-9 and Res concentration （r=－0.969，P=0.03；r=－0.894，P=0.02；r=－0.881，P=0.01）. Conclusion Resveratrol could inhibit the proliferation and induce apoptosis of human gastric cancer HGC27 cells, which may be related to the expression up-regulation of PTEN and phosphorylation inhibition of Akt. Resveratrol could also regulate the expression of VEGF and MMP-9 to inhibit the proliferation and apoptosis of HGC27 cells.

Key words: resveratrol, HGC27 cell, apoptosis, PTEN, p-Akt