›› 2011, Vol. 31 ›› Issue (7): 962-.doi: 10.3969/j.issn.1674-8115.2011.07.020

• Original article (Clinical research) • Previous Articles     Next Articles

Effects of various stimuli on IL-4 release of umbilical cord blood-derived basophils from neonates of mothers with allergic asthma

YANG Ling1, XU Yi-ping2, YAO Su-hang2, XIONG Ying3, ZHU Jie4, WANG Ke-min5   

  1. 1.Department of Geriatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China；2.Allergy Center, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China；3.Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China；4.Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China；5.Department of Biochemistry and Molecular Biology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2010MS12

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Abstract:

Objective To investigate the effects of various stimuli on interleukin-4 (IL-4) release of umbilical cord blood-derived basophils from neonates of mothers with allergic asthma. Methods Samples of umbilical cord blood from neonates of mothers with allergic asthma and healthy mothers were divided into asthma group （n=12） and normal control group （n=16）. Basophils were sorted and purified by flow cytometry, and the cell viability was identified by trypan blue staining. The purified basophils were incubated with ACM buffer (negative control), peptidoglycan (PGN), Dermatophagoides farinae and mannitol respectively, and the mass concentrations of IL-4 in the supernatants of culture fluid were detected by ELISA. Results The result of cell sorting indicated that the purity of basophils was (95.64±3.15)%, with the recovery rate of (60.22±7.18)%, and trypan blue staining revealed that the cell viability was (99.4±0.89)%. Compared with negative control, the mass concentrations of IL-4 in the supernatants of culture fluid significantly increased after stimulation by PGN, Dermatophagoides farinae and mannitol in asthma group （P<0.05 or P<0.01）. After stimulation by PGN and mannitol, the mass concentrations of IL-4 in the supernatants of culture fluid in asthma group were significantly higher than those in normal control group （P<0.05 and P<0.01）. Conclusion PGN, Dermatophagoides farinae and mannitol may stimulate the IL-4 release of umbilical cord blood-derived basophils from neonates of mothers with allergic asthma, induce differentiation of Th2 cells and participate in the pathogenesis of asthma.

Key words: allergic asthma, basophil, umbilical cord blood, interleukin-4