›› 2011, Vol. 31 ›› Issue (11): 1583-.doi: 10.3969/j.issn.1674-8115.2011.11.017

• Original article (Clinical research) • Previous Articles     Next Articles

Characterization of |rpsL and rrs genes of aminoglycoside-resistant clinical isolates of Mycobacterium tuberculosis in Wuhan

WEN Zi-lu1,2, SHEN Jian-guo3, ZHANG Chao-bao1, YU Xiao-li1, CHEN Ping1, TANG Shen-jie4, GUO Xiao-kui2, ZHANG Shu-lin2   

  1. 1.School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China；2.Department of Medical Microbiology and Parasitology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；3.Nanyang Institute of Technology, Nanyang 473004, China；4.Department of |Tuberculosis, the Pulmonary Hospital Affiliated to Tongji University, Shanghai 200433, China
  • Online:2011-11-28 Published:2011-11-29
  • Supported by:

    National Key Technology Program in the 11th Five-Year Plan of China, 2009ZX10003-017, 2009ZX10004-313；Graduate Innovation Foundation of Wuhan Polytechnic University in 2010, 2010cx016

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Abstract:

Objective To characterize the rpsL and rrs genes of aminoglycoside-resistant clinical isolates of Mycobacterium tuberculosis in Wuhan. Methods Sixty-nine clinical isolates of Mycobacterium tuberculosis were collected from Wuhan Medical Treatment Center, including 35 streptomycin (SM)resistance isolates (4 were also resistant to kanamycin) and 34  isolates susceptible to SM (20 were pan-susceptible). Mutation characteristics of rpsL and rrs genes were analyzed with PCR direct sequencing. Results Of the 35 SM-resistant clinical isolates, 26 (74.3%) carried mutations in rpsL gene, with rpsL Lys43Arg and Lys88Arg being the most common rpsL mutations, and one new mutation in codon 88 of rpsL (Lys88Glu) was detected. Four types of mutations in rrs gene in 13 resistant isolates (37.1%) including A1401G (17.1%, 6/35), A514C (14.2%, 5/35), G1487A (2.9%, 1/35) and C517T (2.9%, 1/35) were revealed, and one pan-susceptible  isolate presenting C1029T mutation in rrs gene was detected. The mutations in rrs gene were only found in 3 kanamycin-resistant isolates, and G1487A mutation was detected in rrs gene, which was first reported. Conclusion Aminoglycoside-resistance of Mycobacterium tuberculosis is related to rpsL and rrs gene mutations, and the characteristics of these genes are diverse due to geographical differences.

Key words: Mycobacterium tuberculosis, aminoglycoside, drug resistance, rpsL gene, rrs gene