›› 2013, Vol. 33 ›› Issue (2): 249-.doi: 10.3969/j.issn.1674-8115.2013.02.025

• Technique and method • Previous Articles     Next Articles

Culture and identification of primary neurons isolated from embryonic ICR mice

CHEN Xue-song1,2, MA Ji1, XUE Yan1, ZENG Fan-yi1,2   

  1. 1.Department of Developmental Biology, Institute of Medical Science, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China； 2.Key Laboratory of Medical Embryo Molecular Biology, the Ministry of Health, and Shanghai Laboratory of Embryo and Reproduction Engineering, Shanghai Children´s Hospital, Shanghai Jiaotong University, Shanghai 200040, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    National Natural Science Foundation of China, 81125003, 30900259； National High-Tech Research and Development Program of China, 863 Program, 2011AA020116； Shanghai Science and Technology Committee Foundation, 12XD1406500, 10140900200； National Basic Research Program of China, 973 Program, 2010CB945200

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Abstract:

Objective To establish a favorable primary culture technique for neurons isolated from embryonic ICR mouse cortical tissues. Methods The cortex of embryonic ICR mice aged 13.5 d was isolated, mechanically dissected and digested, and was proceeded to culture. The morphology of neurons was observed, and PCR and Western blotting were applied to identify the expression of Tuj1 and Map2 gene and protein in neurons. Results Cells grew well, with distinct cell body and surrounding bright halation, and there was typical nerve fiber network of synapses. The isolated and cultured cells were confirmed as neurons by PCR and Western blotting. Conclusion This technique is an easy and practical tool for the primary culture of embryonic ICR mouse cortical neurons with high purity.

Key words: neuron, primary culture, embryonic ICR mouse, disease model