›› 2011, Vol. 31 ›› Issue (6): 788-.doi: 10.3969/j.issn.1674-8115.2011.06.023

• 论著(临床研究) • 上一篇    下一篇

新型稳定中性电位水的理化性质及对院内感染常见多重耐药菌的灭活作用

王文奎1, 李作臻2, 张 锋3, 倪语星4, 韩立中4   

  1. 1.上海交通大学 医学院附属瑞金医院灼伤整形科, 上海 200025; 2.青岛市城阳区人民医院检验科, 青岛 266100; 3.中国人民武装警察部队上海市总队医院放射科, 上海 201103; 4.上海交通大学 医学院附属瑞金医院临床微生物科, 上海 200025
  • 出版日期:2011-06-28 发布日期:2011-06-27
  • 通讯作者: 韩立中, 电子信箱: lizhong_han@yahoo.com。
  • 作者简介:王文奎(1965—), 男, 副主任医师, 硕士;电子信箱: wenkuiwang@hotmail.com。
  • 基金资助:

    上海市自然科学基金(09ZR1419200)

Physical and chemical properties of novel stabilized neutral potential water and its antimicrobial effect on common multi-drug resistant pathogens isolated from wards

WANG Wen-kui1, LI Zuo-zhen2, ZHANG feng3, NI Yu-xing4, HAN Li-zhong4   

  1. 1.Department of Burns, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;2.Department of Medical Laboratory, Qingdao Chengyang People's Hospital, Qingdao 266100, China; 3.Department of Radiology Shanghai Fire Corps Hospital, Chinese People's Armed Police Force, Shanghai 201103, China;4.Department of Clinical Microbiology, Ruijing Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-06-28 Published:2011-06-27
  • Supported by:

    Shanghai Natural Science Foundation, 09ZR1419200

摘要:

目的 评估一种新型稳定中性电位水的理化性质,观察其对烧伤科院内感染常见多重耐药病原菌的灭活作用。方法 选择新鲜配制(配制1个月)和保存8个月的新型稳定中性电位水作为待测样液,以有效氯含量、氧化还原电位(ORP)值和pH值等指标评估理化性质,观察长时间保存对其理化性质的影响;采用活菌培养计数的方法观察烧伤科院内感染常见的多重耐药病原菌(耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和鲍曼不动杆菌流行克隆株)在添加新型稳定中性电位水的培养皿上的菌落生长情况,以无菌生理盐水代替中性电位水作为阳性对照进行平行试验。结果 新鲜配制和保存8个月的新型稳定中性电位水待测样液的pH值为6.0~7.0,有效氯含量为50~100 mg/L,ORP值>800 mV,理化性质无明显变化。活菌培养计数结果显示:添加新鲜配制和保存8个月的新型稳定中性电位水的培养皿上均未见菌落生长。结论 新型稳定中性电位水的刺激性小,理化性质稳定,灭菌效果理想,可作为烧伤科环境及与患者密切接触物品的消毒剂。

关键词: 稳定中性电位水, 理化性质, 多重耐药病原菌, 活菌培养计数

Abstract:

Objective To evaluate the physical and chemical properties of a novel stabilized neutral potential water, and observe its antimicrobial effect on common multidrug resistant pathogens isolated from burn wards. Methods Novel neutral potential water samples freshly prepared (prepared within 1 month) and preserved for 8 months were selected, parameters of effective chlorine content, oxidation-reduction potential (ORP) value and pH value were employed to evaluate the physical and chemical properties, and investigate the effects of long-term preservation on the physical and chemical properties. Colony growth of common multi-drug resistant pathogens (methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from burn wards in culture media with novel stabilized neutral potential water was observed by living bacterial counting, and sterilized normal saline was used to substitute neutral potential water as positive control for parallel test. Results The effective chlorine content, ORP value and pH value of novel stabilized neutral potential water samples freshly prepared and preserved for 8 months were 50 to 100 mg/L, >800 mV and 6.0 to 7.0 respectively, and there was no significant difference in physical and chemical properties between freshly prepared sample and that preserved for 8 months. Living bacterial counting revealed that no colony growth was observed in culture media with novel stabilized neutral potential water freshly prepared and preserved for 8 months. Conclusion Novel stabilized neutral potential water has less side effects on skin, stable physical and chemical properties and favorable antimicrobial effect, and can be an ideal disinfectant in burn wards.

Key words: stabilized neutral potential water, physical and chemical properties, multi-drug resistant pathogens, living bacterial counting