上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (1): 30-.doi: 10.3969/j.issn.1674-8115.2013.01.006

• 论著（基础研究） • 上一篇    下一篇

肝癌荷瘤小鼠肥大细胞前体数量和表型的变化特点

宋传旺1, 廖生俊2, 张桂梅2, 冯作化2   

  1. 1.蚌埠医学院 免疫学教研室, 蚌埠 233030； 2.华中科技大学 同济医学院生物化学与分子生物学系, 武汉 430030
  • 出版日期:2013-01-28 发布日期:2013-02-06
  • 作者简介:宋传旺（1972—）, 男, 副教授, 博士, 硕士生导师；电子信箱: chuanwangsong@163.com。

Characteristics of number and phenotype of mast cell precursors in hepatoma-bearing mice

SONG Chuan-wang1, LIAO Sheng-jun2, ZHANG Gui-mei2, FENG Zuo-hua2   

  1. 1.Department of Immunology, Bengbu Medical College, Bengbu 233030, China；2.Department of Biochemistry &|Molecular Biology, Tongji Medical College, Huazhong University of Science &|Technology, Wuhan 430030, China
  • Online:2013-01-28 Published:2013-02-06

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摘要：

目的 研究肝癌荷瘤小鼠肥大细胞前体的数量和表型的变化特点。方法 建立H22肝癌荷瘤小鼠模型，获取骨髓、脾脏及肿瘤组织并制备单细胞悬液，进行荧光抗体染色，流式细胞术检测骨髓中粒-单核细胞前体（GMP）、脾脏中肥大细胞和嗜碱性粒细胞共同前体（BMCP）和外周肿瘤组织中肥大细胞前体的比例，以及后两者内源性危险相关模式分子（DAMP）的识别受体Toll样受体2（TLR2）的表达情况。结果 肝癌荷瘤小鼠骨髓细胞中GMP、脾脏细胞中BMCP和外周肿瘤组织中肥大细胞前体的比例均显著高于正常对照小鼠（P<0.05）。与正常小鼠比较，荷瘤小鼠脾脏BMCP中TLR2表达阳性细胞的比例无明显增高（P>0.05），但外周肿瘤组织肥大细胞前体中TLR2表达阳性细胞的比例显著升高（P<0.05）。结论 H22肝癌荷瘤小鼠肥大细胞动员明显增强，表现为骨髓、髓外器官和外周肿瘤组织中肥大细胞前体的比例显著升高。髓外器官阶段BMCP中TLR2的表达无明显变化，其变化主要发生在外周肿瘤组织阶段的肥大细胞前体。

关键词: 肥大细胞, 肥大细胞前体, 粒-单核细胞前体, 肥大细胞和嗜碱性粒细胞共同前体, Toll样受体2

Abstract:

Objective To investigate the changing characteristics of number and phenotype of mast cell precursors in hepatoma-bearing mice. Methods H22 hepatoma-bearing mouse model was established. Single cells were prepared from bone marrow, spleen and peripheral normal and tumor tissues. Flow cytometry was employed to analyze the proportions of granulocyte/monocyte precursors (GMP) in bone marrow cells, basophil-mast cell progenitors (BMCP) in spleen cells, and mast cell precursors in peripheral normal and tumor tissues. In addition, the expression of Toll-like receptor-2 (TLR2) in BMCP and MCP was determined by flow cytometry. Results The proportions of GMP in bone marrow cells, BMCP in spleen cells and mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice were significantly higher than those in normal mice (P＜0.05). There was no significant difference in proportions of TLR2-positive cells in BMCP in spleen between hepatoma-bearing mice and normal mice (P＞0.05), but the proportion of TLR2-positive cells in mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice was significantly higher than that in normal mice (P＜0.05). Conclusion Mobilization of mast cells is significantly increased and there are higher proportions of mast cell precursors in bone marrow, spleen and peripheral tumor tissues in H22 hepatoma-bearing mice. But there is no significant change in expression of TLR2 in BMCP, and the change mainly takes place in mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice.

Key words: mast cells, mast cell precursors, granulocyte/monocyte precursors, basophil-mast cell progenitors, Toll-like receptor-2