上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (3): 377-.doi: 10.3969/j.issn.1674-8115.2013.03.025

• 短篇论著 • 上一篇    下一篇

一起副溶血性弧菌食物中毒的病原学检测与分析

徐秋芳1, 童 锐1, 费 琼1, 陈 敏2, 陈洪友2, 高红梅1, 马 英1   

  1. 1.上海市青浦区疾病预防控制中心, 上海 201700； 2.上海市疾病预防控制中心, 上海 200336
  • 出版日期:2013-03-28 发布日期:2013-03-29
  • 作者简介:徐秋芳（1985—）, 女, 检验技师, 硕士； 电子信箱: qiufang588@yahoo.com.cn。

Pathogen detection and analysis of one case of food poisoning caused by Vibrio parahemolyticus

XU Qiu-fang1, TONG Rui1, FEI Qiong1, CHEN Min2, CHEN Hong-you2, GAO Hong-mei1, MA Ying1   

  1. 1.Center for Disease Control and Prevention of Qingpu District, Shanghai 201700, China； 2.Center for Disease Control and Prevention of Shanghai, Shanghai 200336, China
  • Online:2013-03-28 Published:2013-03-29

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摘要：

目的 调查一起家庭婚宴中发生的食物中毒事件，运用脉冲场凝胶电泳（PFGE）对所分离的菌株进行同源性分析，为溯源提供依据。方法 调查分析该起事件的流行病学特点，采集患者和食品从业人员肛拭样本及剩余食品和环节样本，采用传统细菌检测的方法进行常规鉴定和血清分型后，对所分离菌株进行PFGE分子分型，用BioNumerics软件对PFGE分型图谱进行聚类分析。结果 本次事件报告确诊发病人数为28例，共检出12株副溶血性弧菌，其中从患者肛拭样本中分离出10株，剩余食品样本中检出2株，血清型均为O4:K8，菌株经PFGE分子分型和聚类分析得到3种带型，3型之间只有2~ 4条电泳条带的差异，具有高度相似性。结论 此起食物中毒事件由PFGE 型别副溶血性弧菌污染食物引起。

关键词: 副溶血性弧菌, 脉冲场凝胶电泳, 食物中毒, 同源性分析

Abstract:

Objective To investigate a food poisoning event in a wedding dinner, and use pulsed field gel electrophoresis (PFGE) technology with isolated strains for homology analysis. Methods The epidemiological characteristics of the case of food poisoning were investigated. The anal swabs of patients and cooks, the residual food samples and link samples were collected, and were identified and serotyped by the traditional bacterial detection. The isolated strains were analysed by PFGE molecular technology, and cluster analysis of PFGE classification photographs was performed by BioNumerics software. Results There were 28 confirmed cases reported, and 12 strains of Vibrio parahemolyticus were detected from 10 anal swabs and 2 residual food samples. The serotype of these strains was O4:K8, and 3 band types were identified by PFGE molecular technology and cluster analysis. These 3 band types were extremely similar, only with differences in 2 to 4 electrophoretic bands. Conclusion This case of food poisoning is caused by Vibrio parahemolyticus.

Key words: Vibrio parahemolyticus, pulsed field gel electrophoresis, food poisoning, homology analysis