上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

双导管法建立兔胸下段食管狭窄模型

刘 洋，刘惠东，马周瑞，陈 笋，孙 锟   

  1. 上海交通大学 医学院附属新华医院小儿心脏中心， 上海 200092
  • 出版日期:2014-10-28 发布日期:2014-10-28
  • 通讯作者: 孙 锟, 电子信箱: drsunkun@xinhuamed.com。
  • 作者简介:刘 洋(1990—) ， 女， 硕士生; 电子信箱: liuyang20092008@126.com。
  • 基金资助:

    国家自然科学基金(81070134，30772349); 上海市科委产学研医合作项目（13DZ1942705）

Establishment of rabbit model of esophageal stenosis in lower thorax by two catheter method

LIU Yang, LIU Hui-dong, MA Zhou-rui, CHEN Sun, SUN Kun   

  1. Department of Pediatric Heart Center， Xinhua Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200092， China
  • Online:2014-10-28 Published:2014-10-28
  • Supported by:

    National Natural Science Foundation of China, 81070134, 30772349; Production-Study-Research-Medical Cooperation Projects, Science and Technology Commission of Shanghai Municipality, 13DZ1942705

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摘要：

目的 探索致食管狭窄的NaOH溶液最佳浓度，建立兔胸下段食管腔狭窄的动物模型。方法 将20只成年兔随机分为实验组（A、B、C组）和对照组,每组各5只。X线监测下，实验组（A、B、C组）及对照组分别经导管注入1%、4%、10%NaOH 溶液和生理盐水各1.5 mL。观察动物精神状态及食欲等，每周监测体质量1次，每2周行1次食管造影检查。4周后处死动物，取出胸下段食管，进行病理学检查。结果 实验A组动物食管未形成明显狭窄；实验C组动物均于伤后72 h内死亡。实验B组动物均形成食管Ⅱ级烧伤，大体形态可见管腔缩窄,食管狭窄指数升高；免疫组织化学观察可见黏膜层缺损、黏膜下层胶原沉积；食管组织羟脯氨酸含量、组织病理学评分及体质量与对照组及实验A组比较，差异均有统计学意义（P<0.01）。结论 在X线监测下采用4% NaOH溶液烧灼食管，可成功建立兔食管良性狭窄模型。

关键词: 食管狭窄, 双导管, NaOH溶液, 最佳浓度

Abstract:

Objective To explore the optimal concentration of NaOH solution that can cause the esophageal stenosis and establish the animal model of esophageal stenosis in lower thorax of rabbits. Methods Twenty adult rabbits were randomly divided into the experiment groups (group A, B, and C) and control group. Each group consisted of 5 rabbits. Under fluoroscopy monitoring, 1.5 mL of 1%, 4%, and 10% NaOH solution and normal saline were given to the experiment groups (group A, B, and C) and control group by catheters, respectively. The mental status and appetite of rabbits were observed. The body mass was weighted once a week and the esophagogram was conducted every 2 weeks. Rabbits were sacrificed after 4 weeks and the esophagus in lower thorax was excised for the pathological examination. Results No obvious esophageal stenosis was found in rabbits of group A. All rabbits of group C died within 72 h after experiment. Seconddegree burn injury and esophageal stenosis were observed in rabbits of group B. Morphological manifestations indicated that stricture index of the esophagus increased. Immunohistochemical observations showed mucous layer defects and accumulation of submucosal collagen. The differences of the hydroxyproline level, histopathologic score, and body mass of group B were significantly different to the control group and group A (P<0.01). Conclusion The rabbit model of benign esophageal stricture can be established successfully by burning the esophagus with 4% NaOH solution under the fluoroscopy monitoring.

Key words: esophageal stenosis, two catheters, NaOH solution, optimal concentration