上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

雌激素缺乏对骨关节炎中Notch信号的影响

付超，李勇，李立峰，肖婷婷，马翔宇，张碧   

  1. 重庆医科大学附属口腔医院口腔与颌面外科，口腔疾病与生物医学重庆市重点实验室，重庆市高校市级口腔生物医学工程重点实验室，重庆 401147
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 张碧，电子信箱：573879079@qq.com。
  • 作者简介:付超（1990—），男，硕士生；电子信箱：747965990@qq.com。
  • 基金资助:

    重庆市教委科学技术研究项目(KJ1400216)

Effects of the estrogen deficiency on Notch signaling pathway in osteoarthritis

FU Chao, LI Yong, LI Li-feng, XIAO Ting-ting, MA Xiang-yu, ZHANG Bi   

  1. Department of Oral and Maxillofacial Surgery, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    Science and Technology Research Project of Chongqing Municipal Education Commission，KJ1400216

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摘要：

目的 ·通过雌激素缺乏-骨关节炎模型探究雌激素缺乏对骨关节炎中Notch信号表达的影响。方法 · 20只雌性SD大鼠随机分为实验组（摘除双侧卵巢）和对照组（假手术处理）。每组再均分骨关节炎亚组（膝关节腔内注射胶原蛋白酶）和非骨关节炎亚组（膝关节腔内注射生理盐水）。6周后取关节组织标本，观察关节软骨形态学改变，免疫组织化学法检测软骨组织中基质金属蛋白酶1（MMP-1）、白介素1β（IL-1β）、Ⅱ型胶原蛋白（Col Ⅱ)和Notch2的表达。结果 ·与对照-骨关节炎亚组比较，实验-骨关节炎亚组大鼠的关节软骨的退变较为严重；软骨组织中MMP-1、IL-1β表达较高，而ColⅡ和Notch2的表达较低。实验-非骨关节炎亚组与对照-非骨关节炎亚组相关指标的检测结果无明显差异。结论 ·雌激素缺乏能下调骨关节炎大鼠软骨组织中Notch信号表达。

关键词: Notch信号通路, 雌激素缺乏, 骨关节炎

Abstract:

Objective · To investigate the effects of estrogen deficiency on Notch signaling pathway in osteoarthritis via an estrogen deficiency-osteoarthritis model. Methods · Twenty female SD rats were randomly assigned to the experimental group (undergoing bilateral ovariectomy) and the control group (sham operation). Each group was further divided into the osteoarthritis subgroup (injection of collagenase into the articular cavity) and the non-osteoarthritis subgroup (injection of normal saline into the articular cavity). Knee specimens were collected after 6 weeks and the morphological changes of the articular cartilage were observed. Expressions of interleukin-1β (IL-1β), matrix metalloproteinases-1 (MMP-1), type Ⅱ collagen (Col Ⅱ), and Notch2 were measured with immunohistochemistry. Results · The degeneration of articular cartilage was more severe in the experimental-osteoarthritis subgroup than in the control-osteoarthritis subgroup. The expressions of IL-1β and MMP-1 in the experimental-osteoarthritis subgroup were higher and the expressions of Col Ⅱ and Notch2 were lower. The differences in measurement results of related indexes between the experimental-non-osteoarthritis subgroup and the control-non-osteoarthritis subgroup were not significant. Conclusion · The estrogen deficiency can down-regulate the expression of Notch signaling in cartilage tissues of rats with osteoarthritis.

Key words: Notch signaling pathway, estrogen deficiency, osteoarthritis