上海交通大学学报(医学版)

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基质辅助激光解析电离飞行时间质谱检测β-内酰胺酶的研究进展

余佳佳,俞静,刘瑛   

  1. 上海交通大学 医学院附属新华医院检验科,临床微生物实验室,上海 200092
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 刘瑛,电子信箱:lywjw0129@163.com。
  • 作者简介:余佳佳(1992—),女,硕士生;电子信箱:yujiajia0302@163.com。
  • 基金资助:

    上海申康医院发展中心临床辅助科室能力建设项目(SHDC22014003)

Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase

YU Jia-jia, YU Jing, LIU Ying   

  1. Department of Clinical Microbiology, Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    Shanghai Shenkang Hospital Development Center Clinical Auxiliary Departments Capacity Building Project,SHDC22014003

摘要:

基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)是一种检测微生物的新型工具,不仅可用于微生物的快速鉴定,而且还可以检测细菌的耐药机制。产
β-内酰胺酶是革兰阴性杆菌耐β-内酰胺类抗菌药物的主要耐药机制,目前利用MALDI-TOF MS快速检测细菌β-内酰胺酶的技术已被广泛报道,方法主要有检测
β-内酰胺酶水解β-内酰胺类抗菌药物的活性,直接检测β-内酰胺酶分子、肽段或其相关蛋白以及检测β-内酰胺酶基因的单核苷酸多态性等。该文结合最新的研究成果对MALDI-TOF MS在β-内酰胺酶检测中的应用现状进行综述。

关键词: 基质辅助激光解析电离飞行时间质谱;&beta, -内酰胺酶;水解试验;单核苷酸多态性

Abstract:

Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is an emerging tool for detecting microorganisms. It can be used not only for rapid identification of microorganisms, but also for the research of resistance mechanisms. Producing β-lactamase is the main mechanism for resistance of β-lactam antibiotics in Gram-negative bacilli. Currently using MALDI-TOF MS for rapid detection of bacterial β-lactamase has been widely reported, including detection of β-lactam hydrolysis activity of β-lactamase, direct detection of β-lactamase molecule, peptide and relevant proteins, and detection of the single nucleotide polymorphisms (SNPs) of β-lactamase gene. This paper reviews current application of MALDI-TOF MS to the detection of β-lactamase based on the latest research findings.

Key words: matrix assisted laser desorption ionization time of flight mass spectrometry, β-lactamase, hydrolysis test, single nucleotide polymorphisms