上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

载顺铂纳米脂质体对荷宫颈癌HeLa细胞移植瘤裸鼠的体内作用

高华 1，王云飞 2，王文静 1，张梅莹 1，李青 1，丁莹 1，狄文 1   

  1. 1. 上海交通大学 医学院附属仁济医院妇产科，上海市妇科肿瘤重点实验室，上海 200127；2.济宁医学院附属医院妇科，济宁 272029
  • 出版日期:2017-05-28 发布日期:2017-05-31
  • 通讯作者: 狄文，电子信箱：diwen163@163.com。
  • 作者简介:高华（1970—），女，副主任医师，博士；电子信箱：gaohuash163@163.com。
  • 基金资助:

    上海市卫生和计划生育委员会项目（20134205）

Effects of cisplatin-alginate conjugate liposomes in cervical cancer-bearing nude mice

GAO Hua1, WANG Yun-fei2, WANG Wen-jing1, ZHANG Mei-ying1, LI Qing1, DING Ying1, DI Wen1   

  1. 1.Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Gynecologic Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 2.Department of Gynecology, Affiliated Hospital of Jining Medical University, Jining 272029, China
  • Online:2017-05-28 Published:2017-05-31
  • Supported by:

    Foundation of Shanghai Municipal Commission of Health and Family Planning, 20134205

PDF (PC)

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摘要：

目的 ·建立荷宫颈癌Hela细胞移植瘤裸鼠模型，检测载顺铂纳米脂质体对该模型小鼠的体内作用和安全性。方法 ·构建Hela细胞皮下成瘤裸鼠模型。观察载顺铂纳米脂质体作用后荷瘤裸鼠的一般状态、肿瘤生长情况，测定及比较瘤体大小和质量，称量并比较裸鼠体质量，对表皮生长因子受体（EGFR）在瘤体中的表达进行免疫组化检测。结果 · ① Hela细胞皮下成瘤裸鼠的肿瘤组织中EGFR高表达。②载顺铂纳米脂质体显著抑制荷瘤小鼠皮下肿瘤的生长（P=0.000）。③载顺铂纳米脂质体组（CS-EGF-Lip）的肿瘤体积抑瘤率达80.22%，瘤体质量抑制率达58.60%，抑瘤效果明显优于顺铂组（P=0.000）。④载顺铂纳米脂质体对荷瘤小鼠体质量的影响最小（P=0.000）。结论 ·载顺铂纳米脂质体给药系统较顺铂的抑瘤作用显著，可以更有效、安全地发挥抑制实体瘤瘤体生长的作用。

关键词: 纳米粒, 宫颈癌, 肿瘤生长

Abstract:

Objective · To establish human cervical cancer xenografts in nude mice and investigate the antitumor therapeutic effects and safety of EGF modified cisplatin-alginate conjugate liposomes. Methods · Cervical cancer-bearing mouse model was established by subcutaneously injecting Hela cells in nude mice. General state and xenograft growth of the mice were observed. Tumor volumes, tumor weights, and the body weights of mice during the treatment were recorded and analyzed. The expression levels of EGFR in xenografts were detected by immunohistochemistry. Results · ① EGFR was highly expressed in the xenografts. ② CS-EGF-Lip inhibited the tumor growth effectively (P=0.000). ③ The inhibition rates of tumor volume and tumor weight of CS-EGF-Lip were 80.22% and 58.60% respectively, which were better than those of cisplatin (P=0.000). ④ CS-EGF-Lip had minimal influence on body weight in mice (P=0.000). Conclusion · CS-EGF-Lip has more effective antitumor effects than cisplatin in cervical cancer-bearing mice, which can inhibit tumor growth of solid tumors with enhanced efficacy and safety.

Key words: nanoparticles, cervical cancer, tumor growth