640 nm红光通过 CD26促进角质形成细胞迁移的实验研究

doi:10.3969/j.issn.1674-8115.2019.08.006

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (8): 843-.doi: 10.3969/j.issn.1674-8115.2019.08.006

640 nm红光通过 CD26促进角质形成细胞迁移的实验研究

张闻，傅秀军，姚敏

上海交通大学医学院附属第九人民医院整复外科，上海 200011

出版日期:2019-08-28 发布日期:2019-09-23
通讯作者: 姚敏，电子信箱：my058@vip.sina.com。
作者简介:张闻（1992—），女，硕士生；电子信箱： zw_hayida @163.com。
基金资助:
国家自然科学基金（81272113）

Promotion of 640 nm red light on keratinocyte migration via CD26

ZHANG Wen, FU Xiu-jun, YAO Min

Department of Plastic and Reconstructive Surgery, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Online:2019-08-28 Published:2019-09-23
Supported by:
National Natural Science Foundation of China, 81272113

摘要/Abstract

摘要： 目的 ·初步探究 640 nm红光促进角质形成细胞迁移的机制。方法 ·体外培养人角质形成细胞，根据干预方式不同分为 4组：对照组、 sitagliptin组（抑制 CD26表达）、640 nm红光组（ 8 J/cm2）、640 nm红光 +sitagliptin组。Realtime-PCR检测角质形成细胞中 CD26 mRNA的表达水平， Western blotting检测 CD26蛋白表达情况， Transwell和细胞划痕实验观察角质形成细胞的迁移能力。结果 · Realtime-PCR和 Western blotting结果显示， 640 nm红光组与对照组相比 CD26 mRNA和蛋白表达有所升高，而 sitagliptin组明显低于对照组（均 P<0.05）；Transwell和细胞划痕实验均可观察到 640 nm红光组角质形成细胞迁移能力增强，而 sitagliptin组迁移能力下降。结论 · 640 nm红光照射可促使 CD26表达增加，加快角质形成细胞迁移，从而缩短再上皮化进程。

关键词: 640 nm红光, CD26, 角质形成细胞, 创面修复, 再上皮化

Abstract:

Objective · To investigate the mechanism of 640 nm red light promoting keratinocyte migration preliminarily. Methods · Human keratinocytes were cultured in vitro and assigned to 4 groups according to different interventions, i.e. control group, sitagliptin group (CD26 inhibited), 640 nm red light group (8 J/cm2) and 640 nm red light+sitagliptin group. The levels of CD26 mRNA and protein in keratinocytes were measured with realtime-PCR and Western blotting, respectively. Cell migration was observed with Transwell assay and scratch assay. Results · The level of CD26 mRNA and the of CD26 protein in 640 nm red light group obviously increased compared with control group, while those of sitagliptin group significantly decreased (all P<0.05). Migration ability of keratinocytes increased in 640 nm red light group and decreased in sitagliptin group observedTranswell assay and scratch assay. Conclusion · The of CD26 in keratinocytes is promotedthe 640 nm red light, which can accelerate keratinocyte migration to facilitate re-epithelialization.

Key words: 640 nm red light, CD26, keratinocyte, wound healing, re-epithelialization

中图分类号:

张闻，傅秀军，姚敏. 640 nm红光通过 CD26促进角质形成细胞迁移的实验研究[J]. 上海交通大学学报（医学版）, 2019, 39(8): 843-.

ZHANG Wen, FU Xiu-jun, YAO Min. Promotion of 640 nm red light on keratinocyte migration via CD26[J]. , 2019, 39(8): 843-.

[1]	许培荣, 吴氢凯, 程慧，等. 交联人脱细胞羊膜修复兔阴道黏膜缺损的研究[J]. 上海交通大学学报（医学版）, 2013, 33(8): 1068-.
[2]	缪明远, 牛轶雯, 陆树良. 巨噬细胞活化与创面愈合[J]. , 2011, 31(8): 1189-.
[3]	傅秀军, 石有振, 方勇, 等. 机械损伤对人皮肤角质形成细胞氧化应激和前列腺素E2分泌的影响[J]. , 2011, 31(7): 905-.
[4]	方勇. 创面修复机制及技术研究进展[J]. , 2009, 29(12): 1403-.

640 nm红光通过 CD26促进角质形成细胞迁移的实验研究

Promotion of 640 nm red light on keratinocyte migration via CD26

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